HUS1 Antibody (PACO27473)
- SKU:
- PACO27473
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | HUS1 Antibody (PACO27473) |
抗体コード: | PACO27473 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Checkpoint protein HUS1 protein (2-280AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western blot All lanes: HUS1 antibody at 2µg/ml Lane 1: Hela whole cell lysate Lane 2: 293T whole cell lysate Lane 2: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 32, 30 kDa Observed band size: 32 kDa . | |
Immunofluorescent analysis of A549 cells using PACO27473 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human gastric cancer using PACO27473 at dilution of 1:100. |
バックグラウンド: | Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase β (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. |
シノニム: | Checkpoint protein HUS1 (hHUS1), HUS1 |
UniProt Protein Function: | HUS1: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Belongs to the HUS1 family. |
UniProt Protein Details: | Protein type:DNA repair, damage; Cell cycle regulation Chromosomal Location of Human Ortholog: 7p13-p12 Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus Molecular Function:protein binding Biological Process: regulation of protein amino acid phosphorylation; embryonic development; negative regulation of DNA replication; mitotic cell cycle checkpoint; DNA damage checkpoint; DNA repair; DNA replication; response to DNA damage stimulus; protein amino acid phosphorylation; double-strand break repair via homologous recombination; response to UV |
NCBI Summary: | The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011] |
UniProt Code: | O60921 |
NCBI GenInfo Identifier: | 74735462 |
NCBI Gene ID: | 3364 |
NCBI Accession: | O60921.1 |
UniProt Secondary Accession: | O60921,B4DFI9, |
UniProt Related Accession: | O60921 |
Molecular Weight: | 280 |
NCBI Full Name: | Checkpoint protein HUS1 |
NCBI Synonym Full Names: | HUS1 checkpoint homolog (S. pombe) |
NCBI Official Symbol: | HUS1 |
NCBI Official Synonym Symbols: | hHUS1 |
NCBI Protein Information: | checkpoint protein HUS1; hus1+-like protein |
UniProt Protein Name: | Checkpoint protein HUS1 |
Protein Family: | Checkpoint protein |
UniProt Gene Name: | HUS1 |
UniProt Entry Name: | HUS1_HUMAN |