HAP1 Antibody (PACO62687)
- SKU:
- PACO62687
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | HAP1 Antibody (PACO62687) |
抗体コード: | PACO62687 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, IF |
推奨される希釈: | ELISA:1:2000-1:10000, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Huntingtin-associated protein 1 protein (328-580AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Immunofluorescence staining of SH-SY5Y cells with PACO62687 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Originally identified as neuronal protein that specifically associates with HTT/huntingtin and the binding is enhanced by an expanded polyglutamine repeat within HTT possibly affecting HAP1 interaction properties. Both HTT and HAP1 are involved in intracellular trafficking and HAP1 is proposed to link HTT to motor proteins and/or transport cargos. Seems to play a role in vesicular transport within neurons and axons such as from early endosomes to late endocytic compartments and to promote neurite outgrowth. The vesicular transport function via association with microtubule-dependent transporters can be attenuated by association with mutant HTT. Involved in the axonal transport of BDNF and its activity-dependent secretion; the function seems to involve HTT, DCTN1 and a complex with SORT1. Involved in APP trafficking and seems to faciltate APP anterograde transport and membrane insertion thereby possibly reducing processing into amyloid beta. Involved in delivery of gamma-aminobutyric acid, (GABA(A)) receptors to synapses; the function is dependent on kinesin motor protein KIF5 and is disrupted by HTT with expanded polyglutamine repeat. Involved in regulation of autophagosome motility by promoting efficient retrograde axonal transport. Seems to be involved in regulation of membrane receptor recycling and degradation, and respective signal transduction, including GABA(A) receptors, tyrosine kinase receptors, EGFR, IP3 receptor and androgen receptor. Among others suggested to be involved in control of feeding behavior (involving hypothalamic GABA(A) receptors), cerebellar and brainstem development (involving AHI1 and NTRK1/TrkA), postnatal neurogenesis (involving hypothalamic NTRK2/TrkB), and ITPR1/InsP3R1-mediated Ca(2+) release (involving HTT and possibly the effect of mutant HTT). Via association with DCTN1/dynactin p150-glued and HTT/huntingtin involved in cytoplasmic retention of REST in neurons. May be involved in ciliogenesis. Involved in regulation of exocytosis. Seems to be involved in formation of cytoplasmic inclusion bodies (STBs). In case of anomalous expression of TBP, can sequester a subset of TBP into STBs; sequestration is enhanced by an expanded polyglutamine repeat within TBP. HAP1-containing STBs have been proposed to play a protective role against neurodegeneration in Huntigton disease (HD) and spinocerebellar ataxia 17 (SCA17). |
シノニム: | Huntingtin-associated protein 1 (HAP-1) (Neuroan 1), HAP1, HAP2 HLP1 |
UniProt Protein Function: | HAP1: Associates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat. 4 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 17q21.2-q21.3 Cellular Component: synaptic vesicle; cytoskeleton; mitochondrion; axon; lysosome; endoplasmic reticulum; autophagic vacuole; inclusion body; nucleus; cell junction; actin cytoskeleton Molecular Function:protein binding; brain-derived neurotrophic factor binding Biological Process: positive regulation of neurogenesis; positive regulation of inositol-1,4,5-triphosphate receptor activity; exocytosis; nerve growth factor receptor signaling pathway; positive regulation of synaptic transmission, GABAergic; hypothalamus cell differentiation; retrograde axon cargo transport; regulation of exocytosis; synaptic transmission; protein transport; protein localization; cell projection organization and biogenesis; positive regulation of epidermal growth factor receptor signaling pathway; vesicle transport along microtubule; positive regulation of neurotrophin production; autophagy; cerebellum development; brain development; anterograde axon cargo transport |
NCBI Summary: | Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with huntingtin, with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), and with a hepatocyte growth factor-regulated tyrosine kinase substrate. The interactions with cytoskeletal proteins and a kinase substrate suggest a role for this protein in vesicular trafficking or organelle transport. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
UniProt Code: | P54257 |
NCBI GenInfo Identifier: | 120431743 |
NCBI Gene ID: | 9001 |
NCBI Accession: | NP_001073339.1 |
UniProt Related Accession: | P54257 |
Molecular Weight: | 74 kDa |
NCBI Full Name: | huntingtin-associated protein 1 isoform 3 |
NCBI Synonym Full Names: | huntingtin associated protein 1 |
NCBI Official Symbol: | HAP1 |
NCBI Official Synonym Symbols: | HLP; HAP2; HIP5; hHLP1 |
NCBI Protein Information: | huntingtin-associated protein 1 |
UniProt Protein Name: | Huntingtin-associated protein 1 |
UniProt Synonym Protein Names: | Neuroan 1 |
Protein Family: | Huntingtin-associated protein |
UniProt Gene Name: | HAP1 |
UniProt Entry Name: | HAP1_HUMAN |
Antibodies |
Anti-HAP1 Antibody (CAB16333) |
Secondary Antibody |
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