GSS Antibody (PACO57792)
- SKU:
- PACO57792
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | GSS Antibody (PACO57792) |
抗体コード: | PACO57792 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000 |
反応性: | Human, Mouse, Rat |
免疫原: | Recombinant Human Glutathione synthetase protein (290-418AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: K562 whole cell lysate, Rat kidney tissue, Mouse liver tissue, Mouse kidney tissue. All lanes: GSS antibody at 5.1µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53, 41 kDa. Observed band size: 53 kDa. | |
IHC image of PACO57792 diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO57792 diluted at 1:500 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | cytosol, extracellular exosome, ATP binding, glutathione binding, glutathione synthase activity, identical protein binding, magnesium ion binding, protein homodimerization activity, cellular amino acid, metabolic process, glutathione biosynthetic process |
シノニム: | Glutathione synthetase (GSH synthetase) (GSH-S) (EC 6.3.2.3) (Glutathione synthase), GSS |
UniProt Protein Function: | GSS: Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency); also known as 5- oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system. Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia. Belongs to the eukaryotic GSH synthase family. |
UniProt Protein Details: | Protein type:EC 6.3.2.3; Other Amino Acids Metabolism - glutathione; Ligase Chromosomal Location of Human Ortholog: 20q11.2 Cellular Component: cytosol Molecular Function:protein homodimerization activity; glutathione binding; glycine binding; magnesium ion binding; glutathione synthase activity; ATP binding Biological Process: amino acid metabolic process; response to nutrient levels; nervous system development; response to cadmium ion; xenobiotic metabolic process; glutathione biosynthetic process; response to oxidative stress; response to amino acid stimulus; aging Disease: Glutathione Synthetase Deficiency; Glutathione Synthetase Deficiency Of Erythrocytes, Hemolytic Anemia Due To |
NCBI Summary: | Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq, Jul 2008] |
UniProt Code: | P48637 |
NCBI GenInfo Identifier: | 1346191 |
NCBI Gene ID: | 2937 |
NCBI Accession: | P48637.1 |
UniProt Secondary Accession: | P48637,Q4TTD9, B2R697, B6F210, E1P5P9, |
UniProt Related Accession: | P48637 |
Molecular Weight: | 474 |
NCBI Full Name: | Glutathione synthetase |
NCBI Synonym Full Names: | glutathione synthetase |
NCBI Official Symbol: | GSS |
NCBI Official Synonym Symbols: | GSHS; HEL-S-64p; HEL-S-88n |
NCBI Protein Information: | glutathione synthetase; GSH-S; GSH synthetase; glutathione synthase; epididymis secretory sperm binding protein Li 64p; epididymis secretory sperm binding protein Li 88n |
UniProt Protein Name: | Glutathione synthetase |
UniProt Synonym Protein Names: | Glutathione synthase |
Protein Family: | Glutathione synthetase |
UniProt Gene Name: | GSS |
UniProt Entry Name: | GSHB_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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