DYNLL1 Antibody (PACO27921)
- SKU:
- PACO27921
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | DYNLL1 Antibody (PACO27921) |
抗体コード: | PACO27921 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Dynein light chain 1, cytoplasmic protein (1-89AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western blot All lanes: DYNLL1 antibody at 2µg/ml + EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 11 kDa Observed band size: 11 kDa . | |
Immunohistochemistry of paraffin-embedded human tonsil tissue using PACO27921 at dilution of 1:100. | |
Immunohistochemistry of paraffin-embedded human cervical cancer using PACO27921 at dilution of 1:100. |
バックグラウンド: | Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures. Binds and inhibits the catalytic activity of neuronal nitric oxide synthase. Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1. Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity. |
シノニム: | Dynein light chain 1, cytoplasmic (8 kDa dynein light chain) (DLC8) (Dynein light chain LC8-type 1) (Protein inhibitor of neuronal nitric oxide synthase) (PIN), DYNLL1, DLC1 DNCL1 DNCLC1 HDLC1 |
UniProt Protein Function: | LC8: a highly conserved cytoplasmic protein of the dynein light chain family. Cytoplasmic dyneins are large enzyme complexes. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain. Interacts with and inhibits the activity of neuronal nitric oxide synthase (nNOS). A highly conserved protein, showing 92% amino acid similarity with the nematode homolog. Binding of this protein destabilizes the nNOS dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. |
UniProt Protein Details: | Protein type:Microtubule-binding; Motility/polarity/chemotaxis; Motor Chromosomal Location of Human Ortholog: 12q24.23 Cellular Component: kinetochore; signalosome; microtubule; centrosome; membrane; cytoplasmic dynein complex; mitochondrion; cytoplasm; plasma membrane; nucleus; cytosol Molecular Function:protein C-terminus binding; protein domain specific binding; protein binding; enzyme binding; protein homodimerization activity; nitric-oxide synthase regulator activity; motor activity Biological Process: anatomical structure morphogenesis; viral reproduction; transcription, DNA-dependent; apoptosis; organelle organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; female gamete generation; microtubule-based process; substantia nigra development; regulation of transcription, DNA-dependent; transport; regulation of catalytic activity; negative regulation of phosphorylation; mitotic cell cycle; actin cytoskeleton organization and biogenesis; G2/M transition of mitotic cell cycle |
NCBI Summary: | Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1,200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008] |
UniProt Code: | P63167 |
NCBI GenInfo Identifier: | 52783578 |
NCBI Gene ID: | 8655 |
NCBI Accession: | P63167.1 |
UniProt Secondary Accession: | P63167,Q15701, |
UniProt Related Accession: | P63167 |
Molecular Weight: | 89 |
NCBI Full Name: | Dynein light chain 1, cytoplasmic |
NCBI Synonym Full Names: | dynein, light chain, LC8-type 1 |
NCBI Official Symbol: | DYNLL1 |
NCBI Official Synonym Symbols: | LC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1 |
NCBI Protein Information: | dynein light chain 1, cytoplasmic; 8 kDa dynein light chain; cytoplasmic dynein light polypeptide; dynein, cytoplasmic, light polypeptide 1; protein inhibitor of neuronal nitric oxide synthase |
UniProt Protein Name: | Dynein light chain 1, cytoplasmic |
UniProt Synonym Protein Names: | 8 kDa dynein light chain; DLC8; Dynein light chain LC8-type 1; Protein inhibitor of neuronal nitric oxide synthase; PIN |
UniProt Gene Name: | DYNLL1 |
UniProt Entry Name: | DYL1_HUMAN |