DNASE1 Antibody (PACO51466)
- SKU:
- PACO51466
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | DNASE1 Antibody (PACO51466) |
抗体コード: | PACO51466 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000 |
反応性: | Human, Mouse |
免疫原: | Recombinant Human Deoxyribonuclease-1 protein (78-231AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Mouse brain tissue. All lanes: DNASE1 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 32, 20 kDa. Observed band size: 32 kDa.. | |
IHC image of PACO51466 diluted at 1:600 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity). |
シノニム: | Deoxyribonuclease-1 (EC 3.1.21.1) (Deoxyribonuclease I) (DNase I) (Dornase alfa), DNASE1, DNL1 DRNI |
UniProt Protein Function: | DNASE1: Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Defects in DNASE1 are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the DNase I family. |
UniProt Protein Details: | Protein type:Deoxyribonuclease; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; EC 3.1.21.1 Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: extracellular region; nuclear envelope; nucleus Molecular Function:protein binding; deoxyribonuclease I activity; actin binding; endodeoxyribonuclease activity Biological Process: apoptosis; DNA catabolic process, endonucleolytic Disease: Systemic Lupus Erythematosus |
NCBI Summary: | This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008] |
UniProt Code: | P24855 |
NCBI GenInfo Identifier: | 118919 |
NCBI Gene ID: | 1773 |
NCBI Accession: | P24855.1 |
UniProt Secondary Accession: | P24855,Q14UU9, Q14UV0, B4DV35, |
UniProt Related Accession: | P24855 |
Molecular Weight: | Calculated: 19kDa/31kDaObserved: 37kDa |
NCBI Full Name: | Deoxyribonuclease-1 |
NCBI Synonym Full Names: | deoxyribonuclease I |
NCBI Official Symbol: | DNASE1 |
NCBI Official Synonym Symbols: | DNL1; DRNI |
NCBI Protein Information: | deoxyribonuclease-1; Dornase alfa; DNase I, lysosomal; human urine deoxyribonuclease I |
UniProt Protein Name: | Deoxyribonuclease-1 |
UniProt Synonym Protein Names: | Deoxyribonuclease I; DNase I; INN: Dornase alfa |
Protein Family: | Deoxyribonuclease |
UniProt Gene Name: | DNASE1 |
UniProt Entry Name: | DNAS1_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |