CRYBA1 Antibody (PACO25953)
- SKU:
- PACO25953
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | CRYBA1 Antibody (PACO25953) |
抗体コード: | PACO25953 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human, Mouse |
免疫原: | Recombinant Human Beta-crystallin A3 protein (1-215AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western blot All lanes: CRYBA1 antibody at 6µg/ml Lane 1: Human placenta tissue Lane 2: U251 whole cell lysate Lane 3: U937 whole cell lysate Lane 4: Mouse stomach tissue Lane 5: Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 26, 24 kDa Observed band size: 24 kDa . | |
Immunohistochemistry of paraffin-embedded human heart tissue using PACO25953 at dilution of 1:100. | |
Immunofluorescence staining of A549 cells with PACO25953 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Crystallins are the dominant structural components of the vertebrate eye lens. |
シノニム: | Beta-crystallin A3 [Cleaved into: Beta-crystallin A3, isoform A1, Delta4 form; Beta-crystallin A3, isoform A1, Delta7 form; Beta-crystallin A3, isoform A1, Delta8 form], CRYBA1, CRYB1 |
UniProt Protein Function: | CRYBA1: a major structural protein of the eye lens. A member of the beta/gamma-crystallin family. Defects are the cause of congenital zonular cataract with sutural opacities. Two splice-variant isoforms have been described. |
UniProt Protein Details: | Chromosomal Location of Human Ortholog: 17q11.2 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding Biological Process: visual perception Disease: Cataract 10, Multiple Types |
NCBI Summary: | Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'. [provided by RefSeq, Jul 2008] |
UniProt Code: | P05813 |
NCBI GenInfo Identifier: | 2506317 |
NCBI Gene ID: | 1411 |
NCBI Accession: | P05813.4 |
UniProt Secondary Accession: | P05813,Q13633, Q14CM9, |
UniProt Related Accession: | P05813 |
Molecular Weight: | 23,191 Da |
NCBI Full Name: | Beta-crystallin A3 |
NCBI Synonym Full Names: | crystallin beta A1 |
NCBI Official Symbol: | CRYBA1 |
NCBI Official Synonym Symbols: | CRYB1; CTRCT10 |
NCBI Protein Information: | beta-crystallin A3 |
UniProt Protein Name: | Beta-crystallin A3 |
Protein Family: | Beta-crystallin |
UniProt Gene Name: | CRYBA1 |
UniProt Entry Name: | CRBA1_HUMAN |
Antibodies |
Anti-CRYBA1 Antibody (CAB15267) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |