ATP6V1E1 Antibody (PACO30234)
- SKU:
- PACO30234
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | ATP6V1E1 Antibody (PACO30234) |
抗体コード: | PACO30234 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human V-type proton ATPase subunit E 1 protein (2-224AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western blot All lanes: ATP6V1E1 antibody at 2µg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 27, 24, 23 kDa Observed band size: 36 kDa . | |
Immunofluorescent analysis of Hela cells using PACO30234 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acid, fying a variety of intracellular compartments in eukaryotic cells. |
シノニム: | V-type proton ATPase subunit E 1 (V-ATPase subunit E 1) (V-ATPase 31 kDa subunit) (p31) (Vacuolar proton pump subunit E 1), ATP6V1E1, ATP6E ATP6E2 |
UniProt Protein Function: | ATP6V1E1: Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase E subunit family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Mitochondrial; Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14; Hydrolase Chromosomal Location of Human Ortholog: 22q11.1 Cellular Component: microvillus; mitochondrion; lysosomal membrane; apical plasma membrane; proton-transporting two-sector ATPase complex; cytosol; endosome Molecular Function:hydrogen-exporting ATPase activity, phosphorylative mechanism; protein binding; hydrogen ion transporting ATPase activity, rotational mechanism; ATPase binding Biological Process: interaction with host; proton transport; metabolic process; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport |
NCBI Summary: | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq, Jul 2008] |
UniProt Code: | P36543 |
NCBI GenInfo Identifier: | 47678301 |
NCBI Gene ID: | 529 |
NCBI Accession: | CAG30271.1 |
UniProt Secondary Accession: | P36543,A8MUE4, A8MUN4, |
UniProt Related Accession: | P36543 |
Molecular Weight: | 22,706 Da |
NCBI Full Name: | ATP6E |
NCBI Synonym Full Names: | ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 |
NCBI Official Symbol: | ATP6V1E1 |
NCBI Official Synonym Symbols: | P31; Vma4; ATP6E; ATP6E2; ATP6V1E |
NCBI Protein Information: | V-type proton ATPase subunit E 1 |
UniProt Protein Name: | V-type proton ATPase subunit E 1 |
UniProt Synonym Protein Names: | V-ATPase 31 kDa subunit; p31; Vacuolar proton pump subunit E 1 |
UniProt Gene Name: | ATP6V1E1 |
UniProt Entry Name: | VATE1_HUMAN |