ARID3A Antibody (PACO56110)
- SKU:
- PACO56110
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | ARID3A Antibody (PACO56110) |
抗体コード: | PACO56110 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
反応性: | Human, Mouse |
免疫原: | Recombinant Human AT-rich interactive domain-containing protein 3A protein (26-137AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Mouse liver tissue. All lanes: ARID3A antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 63 kDa. Observed band size: 63 kDa. | |
IHC image of PACO56110 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO56110 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation. |
シノニム: | AT-rich interactive domain-containing protein 3A (ARID domain-containing protein 3A) (B-cell regulator of IgH transcription) (Bright) (Dead ringer-like protein 1) (E2F-binding protein 1), ARID3A, DRIL1 DRIL3 DRX E2FBP1 |
UniProt Protein Function: | Bright: a member of the ARID (AT-rich interaction domain) family of transcription factors. It was found by its homology to the Drosophila dead ringer protein, which is important for normal embryogenesis. Binds a VH promoter proximal site necessary for induced mu-heavy-chain transcription. Binds the minor groove of a restricted ATC sequence that is sufficient for nuclear matrix association. This sequence motif is present in matrix-associating regions (MARS) proximal to the promoter and flanking E mu. Activates E mu-driven transcription by binding these sites. Contributes to localized control of accessibility and therefore nonrandom gene use during V(D)J recombination. |
UniProt Protein Details: | Protein type:Transcription factor Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; nucleolus; lipid raft Molecular Function:protein binding; protein homodimerization activity; chromatin binding Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter |
NCBI Summary: | This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q99856 |
NCBI GenInfo Identifier: | 12230034 |
NCBI Gene ID: | 1820 |
NCBI Accession: | Q99856.2 |
UniProt Related Accession: | Q99856 |
Molecular Weight: | |
NCBI Full Name: | AT-rich interactive domain-containing protein 3A |
NCBI Synonym Full Names: | AT-rich interaction domain 3A |
NCBI Official Symbol: | ARID3A |
NCBI Official Synonym Symbols: | DRIL1; DRIL3; BRIGHT; E2FBP1 |
NCBI Protein Information: | AT-rich interactive domain-containing protein 3A |
UniProt Protein Name: | AT-rich interactive domain-containing protein 3A |
UniProt Synonym Protein Names: | B-cell regulator of IgH transcription; Bright; Dead ringer-like protein 1; E2F-binding protein 1 |
UniProt Gene Name: | ARID3A |
UniProt Entry Name: | ARI3A_HUMAN |