Anti-VDAC1 Antibody (RACO0177)
- SKU:
- RACO0177
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
商品名: | VDAC1 Antibody |
Product SKU: | RACO0177 |
サイズ: | 50ul |
宿主種: | Human |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
反応性: | Human, Mouse, Rat |
免疫原: | A synthesized peptide derived from human VDAC1 |
憲法: | Liquid |
ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
精製方法: | Affinity-chromatography |
抗体のクローン性: | Monoclonal |
アイソタイプ: | Rabbit IgG |
Conjugate: | Non-conjugated |
バックグラウンド: | Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:11845315, PubMed:18755977, PubMed:20230784, PubMed:8420959). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756). |
シノニム: | Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, VDAC1, VDAC |
IHC image of RACO0177 diluted at 1:73.125 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of RACO0177 diluted at 1:73.125 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Western Blot. Positive WB detected in Hela whole cell lysate HepG2 whole cell lysate 293 whole cell lysate Jurkat whole cell lysate HL-60 whole cell lysate LO2 whole cell lysate Raji whole cell lysate Rat heart tissue, Mouse brain tissue. All lanes VDAC1 antibody at 0.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 31 KDa. Observed band size: 31 KDa. |
UniProt Protein Function: | VDAC1: a protein of the eukaryotic mitochondrial porin family. Forms a voltage-dependent anion channel through the mitochondrial outer membrane and also the plasma membrane. The channel allows diffusion of small hydrophilic molecules; it adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. |
UniProt Protein Details: | Protein type:Membrane protein, integral; Channel, misc.; Mitochondrial Chromosomal Location of Human Ortholog: 5q31 Cellular Component: lipid raft; membrane; mitochondrial outer membrane; mitochondrion; myelin sheath; nucleus; plasma membrane; pore complex; synaptic vesicle Molecular Function:porin activity; protein binding; protein complex binding; protein kinase binding; voltage-gated anion channel activity Biological Process: anion transport; apoptosis; behavioral fear response; epithelial cell differentiation; learning; macroautophagy; macromitophagy; mitochondrial calcium ion transport; nerve-nerve synaptic transmission; viral reproduction |
NCBI Summary: | This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.[provided by RefSeq, Sep 2010] |
UniProt Code: | P21796 |
NCBI GenInfo Identifier: | 130683 |
NCBI Gene ID: | 7416 |
NCBI Accession: | P21796.2 |
UniProt Secondary Accession: | P21796,Q5FVE7, Q9UIQ5, Q9UPL0, B3KVK4, D3DQ93, |
UniProt Related Accession: | P21796 |
Molecular Weight: | 30,773 Da |
NCBI Full Name: | Voltage-dependent anion-selective channel protein 1 |
NCBI Synonym Full Names: | voltage dependent anion channel 1 |
NCBI Official Symbol: | VDAC1 |
NCBI Official Synonym Symbols: | PORIN; VDAC-1 |
NCBI Protein Information: | voltage-dependent anion-selective channel protein 1 |
UniProt Protein Name: | Voltage-dependent anion-selective channel protein 1 |
UniProt Synonym Protein Names: | Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31HL; Porin 31HM |
Protein Family: | Porin |
UniProt Gene Name: | VDAC1 |
UniProt Entry Name: | VDAC1_HUMAN |