Anti-TYR Antibody (RACO0332)
- SKU:
- RACO0332
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IF
- Application:
- ELISA
- Conjugation:
- Unconjugated
Description
商品名: | TYR Antibody |
Product SKU: | RACO0332 |
サイズ: | 50ul |
宿主種: | Homo sapiens (Human) |
申し込み: | ELISA, IF |
推奨される希釈: | IF:1:20-1:200 |
反応性: | Human |
免疫原: | A synthesized peptide derived from human Tyrosinase |
憲法: | Liquid |
ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
精製方法: | Affinity-chromatography |
抗体のクローン性: | Monoclonal |
アイソタイプ: | Rabbit IgG |
Conjugate: | Non-conjugated |
バックグラウンド: | This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone. |
シノニム: | Tyrosinase (EC 1.14.18.1) (LB24-AB) (Monophenol monooxygenase) (SK29-AB) (Tumor rejection antigen AB), TYR |
Immunofluorescence staining of MCF7 Cells with RACO0332 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
UniProt Protein Function: | TYR: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone. Increased expression after UVB irradiation. Belongs to the tyrosinase family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Amino Acid Metabolism - tyrosine; EC 1.14.18.1; Membrane protein, integral; Cofactor and Vitamin Metabolism - riboflavin; Oxidoreductase Chromosomal Location of Human Ortholog: 11q14.3 Cellular Component: lysosome; perinuclear region of cytoplasm; cytoplasm; melanosome membrane; integral to membrane; melanosome; Golgi-associated vesicle Molecular Function:protein binding; copper ion binding; protein homodimerization activity; protein heterodimerization activity; monophenol monooxygenase activity Biological Process: cell proliferation; visual perception; thymus development; melanin biosynthetic process from tyrosine; eye pigment biosynthetic process Disease: Albinism, Ocular, With Sensorineural Deafness; Albinism, Oculocutaneous, Type Ib; Albinism, Oculocutaneous, Type Ia; Skin/hair/eye Pigmentation, Variation In, 3 |
NCBI Summary: | The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq, Oct 2008] |
UniProt Code: | P14679 |
NCBI GenInfo Identifier: | 401235 |
NCBI Gene ID: | 7299 |
NCBI Accession: | P14679.3 |
UniProt Secondary Accession: | P14679,Q15675, Q15676, Q15680, Q8TAK4, Q9BYY0, Q9BZX1 |
UniProt Related Accession: | P14679 |
Molecular Weight: | Calculated MW: 42kDa/60kDaObserved MW: 80kDa |
NCBI Full Name: | Tyrosinase |
NCBI Synonym Full Names: | tyrosinase |
NCBI Official Symbol: | TYR |
NCBI Official Synonym Symbols: | ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3 |
NCBI Protein Information: | tyrosinase; LB24-AB; SK29-AB; monophenol monooxygenase; oculocutaneous albinism IA; tumor rejection antigen AB |
UniProt Protein Name: | Tyrosinase |
UniProt Synonym Protein Names: | LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB |
Protein Family: | Tyrosinase |
UniProt Gene Name: | TYR |
UniProt Entry Name: | TYRO_HUMAN |