Anti-Symmetric DiMethyl-Histone H3-R2 抗体 (CAB2373)

抗体名:	Anti-Symmetric DiMethyl-Histone H3-R2 Antibody
抗体コード:	CAB2373
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF IP ChIP ChIPseq
反応性:	Human, Mouse, Rat, Other (Wide Range)
宿主種:	Rabbit
免疫原:	A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3

申し込み:	WB IHC IF IP ChIP ChIPseq
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 ChIPseq 1:20 - 1:100
反応性:	Human, Mouse, Rat, Other (Wide Range)
ポジティブサンプル:	HeLa, NIH/3T3, C6

免疫原:	A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	Email for sequence
遺伝子ID:	8290
Uniprot:	Q16695
セルラーロケーション:	Chromosome, Nucleus
計算された分子量:	15kDa
観察された分子量:	17KDa

同義語:

H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A

バックグラウンド:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

UniProt Protein Function:	HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 1q42 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; DNA binding; histone binding; protein heterodimerization activity Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance
NCBI Summary:	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
UniProt Code:	Q16695
NCBI GenInfo Identifier:	18202512
NCBI Gene ID:	8290
NCBI Accession:	Q16695.3
UniProt Secondary Accession:	Q16695,Q6FGU4, B2R5K3,
UniProt Related Accession:	Q16695
Molecular Weight:
NCBI Full Name:	Histone H3.1t
NCBI Synonym Full Names:	histone cluster 3, H3
NCBI Official Symbol:	HIST3H3
NCBI Official Synonym Symbols:	H3t; H3.4; H3/g; H3FT
NCBI Protein Information:	histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
UniProt Protein Name:	Histone H3.1t
UniProt Synonym Protein Names:	H3/g
UniProt Gene Name:	HIST3H3
UniProt Entry Name:	H31T_HUMAN

	Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R2 antibody (CAB2373). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R2 antibody (CAB2373) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R2 antibody (CAB2373).
	Immunohistochemistry of paraffin-embedded rat liver using Symmetric DiMethyl-Histone H3-R2 antibody (CAB2373) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using Symmetric DiMethyl-Histone H3-R2 antibody (CAB2373) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-Acetyl-Histone H3-K36 Antibody (CAB16077)	Human HIST3H3 (Histone H3.1t) ELISA Kit (HUFI03433)
Anti-Acetyl-Histone H3-K4 Antibody (CAB16078)
Anti-TriMethyl-Histone H3-K27 Antibody (CAB16199)
Anti-Histone H3 Antibody (CAB2348)
Anti-Histone H3 Antibody (CAB2352)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)