Anti-SPP1 Antibody (RACO0523)
- SKU:
- RACO0523
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
商品名: | SPP1 Antibody |
Product SKU: | RACO0523 |
サイズ: | 50ul |
宿主種: | Homo sapiens (Human) |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
反応性: | Human |
免疫原: | A synthesized peptide derived from human Osteopontin |
憲法: | Liquid |
ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
精製方法: | Affinity-chromatography |
抗体のクローン性: | Monoclonal |
アイソタイプ: | Rabbit IgG |
Conjugate: | Non-conjugated |
バックグラウンド: | Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. |
シノニム: | Osteopontin (Bone sialoprotein 1) (Nephropontin) (Secreted phosphoprotein 1) (SPP-1) (Urinary stone protein) (Uropontin), SPP1, BNSP OPN |
IHC image of RACO0523 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. | |
Western Blot. Positive WB detected in 293T whole cell lysate U-251 whole cell lysate U-87 whole cell lysate All lanes Osteopontin Antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 36, 34, 33, 34, 34 kDa. Observed band size: 36 kDa. |
UniProt Protein Function: | osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 4q22.1 Cellular Component: extracellular space; cell projection; apical part of cell; perinuclear region of cytoplasm; extracellular region Molecular Function:protein binding; extracellular matrix binding; cytokine activity Biological Process: osteoblast differentiation; extracellular matrix disassembly; neutrophil chemotaxis; extracellular matrix organization and biogenesis; response to vitamin D; biomineral formation; response to steroid hormone stimulus; negative regulation of collateral sprouting of intact axon in response to injury; positive regulation of bone resorption; decidualization; cell adhesion; inflammatory response; embryo implantation |
NCBI Summary: | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
UniProt Code: | P10451 |
NCBI GenInfo Identifier: | 129260 |
NCBI Gene ID: | 6696 |
NCBI Accession: | P10451.1 |
UniProt Secondary Accession: | P10451,Q15681, Q15682, Q15683, Q4W597, Q567T5, Q8NBK2 Q96IZ1, B2RDA1, |
UniProt Related Accession: | P10451 |
Molecular Weight: | 33,843 Da |
NCBI Full Name: | Osteopontin |
NCBI Synonym Full Names: | secreted phosphoprotein 1 |
NCBI Official Symbol: | SPP1 |
NCBI Official Synonym Symbols: | OPN; BNSP; BSPI; ETA-1 |
NCBI Protein Information: | osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) |
UniProt Protein Name: | Osteopontin |
UniProt Synonym Protein Names: | Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin |
Protein Family: | Osteopontin |
UniProt Gene Name: | SPP1 |
UniProt Entry Name: | OSTP_HUMAN |