Anti-SHMT2 Antibody (CAB1215)[KO Validated]
- SKU:
- CAB1215
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
抗体名: | Anti-SHMT2 Antibody [KO Validated] |
抗体コード: | CAB1215 |
抗体サイズ: | 20uL, 50uL, 100uL |
申し込み: | WB IHC IF IP |
反応性: | Human, Mouse, Rat |
宿主種: | Rabbit |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human SHMT2 (NP_005403.2). |
申し込み: | WB IHC IF IP |
推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 |
反応性: | Human, Mouse, Rat |
ポジティブサンプル: | HeLa, 293T, SW620 |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human SHMT2 (NP_005403.2). |
精製方法: | Affinity purification |
ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
アイソタイプ: | IgG |
順序: | PSPF KHAD IVTT TTHK TLRG ARSG LIFY RKGV KAVD PKTG REIP YTFE DRIN FAVF PSLQ GGPH NHAI AAVA VALK QACT PMFR EYSL QVLK NARA MADA LLER GYSL VSGG TDNH LVLV DLRP KGLD GARA ERVL ELVS ITAN KNTC PGDR SAIT PGGL RLGA PALT SRQF REDD FRRV VDFI DEGV NIGL EVKS KTAK LQDF KSFL LKDS ETSQ RLAN LRQR VEQF ARAF PMPG FDEH |
遺伝子ID: | 6472 |
Uniprot: | P34897 |
セルラーロケーション: | Mitochondrion, Mitochondrion inner membrane, Mitochondrion matrix, mitochondrion nucleoid |
計算された分子量: | 53kDa/54kDa/55kDa |
観察された分子量: | 56KDa |
同義語: | SHMT2, GLYA, HEL-S-51e, SHMT |
バックグラウンド: | This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5, 10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. |
UniProt Protein Function: | SHMT2: Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA. Belongs to the SHMT family. 3 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Amino Acid Metabolism - glycine, serine and threonine; Methyltransferase; Energy Metabolism - methane; Cofactor and Vitamin Metabolism - one carbon pool by folate; Mitochondrial; EC 2.1.2.1; Other Amino Acids Metabolism - cyanoamino acid Chromosomal Location of Human Ortholog: 12q12-q14 Cellular Component: microtubule cytoskeleton; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; mitochondrial intermembrane space Molecular Function:L-allo-threonine aldolase activity; identical protein binding; amino acid binding; glycine hydroxymethyltransferase activity; chromatin binding; pyridoxal phosphate binding Biological Process: L-serine biosynthetic process; positive regulation of cell proliferation; one-carbon compound metabolic process; glycine biosynthetic process from serine; protein homotetramerization |
NCBI Summary: | This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
UniProt Code: | P34897 |
NCBI GenInfo Identifier: | 6226865 |
NCBI Gene ID: | 6472 |
NCBI Accession: | P34897.3 |
UniProt Related Accession: | P34897 |
Molecular Weight: | ~ 56kDa |
NCBI Full Name: | Serine hydroxymethyltransferase, mitochondrial |
NCBI Synonym Full Names: | serine hydroxymethyltransferase 2 |
NCBI Official Symbol: | SHMT2 |
NCBI Official Synonym Symbols: | GLYA; SHMT; HEL-S-51e |
NCBI Protein Information: | serine hydroxymethyltransferase, mitochondrial |
UniProt Protein Name: | Serine hydroxymethyltransferase, mitochondrial |
UniProt Synonym Protein Names: | Glycine hydroxymethyltransferase; Serine methylase |
UniProt Gene Name: | SHMT2 |
UniProt Entry Name: | GLYM_HUMAN |