Anti-Phospho-RPS6KA1 (T359+S363) Antibody (RACO0049)

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SKU:
RACO0049
Product type:
Recombinant Antibody
Reactivity:
Human
Host Species:
Human
Isotype:
IgG
Application:
IP
Application:
WB
Application:
IF
Application:
ELISA
Conjugation:
Unconjugated
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Description

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商品名:Phospho-RPS6KA1 (T359+S363) Antibody
Product SKU:RACO0049
サイズ:50ul
宿主種:Human
申し込み:ELISA, WB, IF, IP
推奨される希釈:WB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000
反応性:Human
免疫原:A synthesized peptide derived from human Phospho-RPS6KA1 (T359+S363)
憲法:Liquid
ストレージバッファ:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
精製方法:Affinity-chromatography
抗体のクローン性:Monoclonal
アイソタイプ:Rabbit IgG
Conjugate:Non-conjugated
バックグラウンド:Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB (By similarity). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration (PubMed:26158630).
シノニム:Ribosomal protein S6 kinase alpha-1, S6K-alpha-1, 90 kDa ribosomal protein S6 kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP kinase-activated protein kinase 1a, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a, Ribosomal S6 kinase 1, RSK-1, RPS6KA1, MAPKAPK1A, RSK1
Product ImageImmunofluorescence staining of Hela cells with RACO0049 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Product ImageImmunoprecipitating Phospho-RPS6KA1 in Hela whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of RACO0049 in Hela whole cell lysate For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: RACO0049 (3µg) + Hela whole cell lysate (1mg). Lane 3: Hela whole cell lysate (20µg).
Product ImageWestern Blot. Positive WB detected in HepG2 whole cell lysate treated with EGF or not). All lanes Phospho-RPS6KA1 antibody at 0.69µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 90 KDa. Observed band size: 90 KDa.
UniProt Protein Function:p90RSK: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.
UniProt Protein Details:

Protein type:EC 2.7.11.1; Protein kinase, AGC; Kinase, protein; Translation; Protein kinase, Ser/Thr (non-receptor); AGC group; RSK family; RSK subfamily

Chromosomal Location of Human Ortholog: 1p

Cellular Component: nucleoplasm; cytoplasm; spindle; cytosol; nucleus

Molecular Function:protein serine/threonine kinase activity; protein binding; caspase inhibitor activity; protein serine/threonine/tyrosine kinase activity; magnesium ion binding; ATP binding

Biological Process: regulation of transcription in response to stress; axon guidance; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; regulation of translation in response to stress; toll-like receptor 3 signaling pathway; negative regulation of caspase activity; cell cycle; signal transduction; protein amino acid phosphorylation; positive regulation of cell growth; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; synaptic transmission; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; positive regulation of cell differentiation; toll-like receptor 4 signaling pathway; negative regulation of apoptosis

NCBI Summary:This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:Q15418
NCBI GenInfo Identifier:20178306
NCBI Gene ID:6195
NCBI Accession:Q15418.2
UniProt Secondary Accession:Q15418,Q5SVM5, Q5SVM8, Q5SVM9, Q96C05, Q9BQK2, A6NGG4 A8K9K7, B2RDY8, B7Z5J0,
UniProt Related Accession:Q15418
Molecular Weight:72,698 Da
NCBI Full Name:Ribosomal protein S6 kinase alpha-1
NCBI Synonym Full Names:ribosomal protein S6 kinase, 90kDa, polypeptide 1
NCBI Official Symbol:RPS6KA1  
NCBI Official Synonym Symbols:RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A  
NCBI Protein Information:ribosomal protein S6 kinase alpha-1; 90 kDa ribosomal protein S6 kinase 1; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; RSK-1; S6K-alpha 1; S6K-alpha-1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); p90-RSK 1; p90RSK1; p90S6K; ribosomal S6 kinase 1; ribosomal protein S6 kinase alpha 1
UniProt Protein Name:Ribosomal protein S6 kinase alpha-1
UniProt Synonym Protein Names:90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; Ribosomal S6 kinase 1; RSK-1
Protein Family:Ribosomal protein S6 kinase
UniProt Gene Name:RPS6KA1  
UniProt Entry Name:KS6A1_HUMAN
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