Anti-Phospho-NDRG1-T346 pAb Antibody (CABP0792)

(No reviews yet) Write a Review
SKU:
CABP0792
Product type:
Antibody
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€0.00
Frequently bought together:

Description

system_update_altデータシートsystem_update_alt安全性データシート
抗体名:Anti-Phospho-NDRG1-T346 Antibody
抗体コード:CABP0792
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB
反応性:Human, Mouse
宿主種:Rabbit
免疫原:A synthetic phosphorylated peptide around T346 of human NDRG1 (NP_001128714.1).
申し込み:WB
推奨希釈:WB 1:500 - 1:2000
反応性:Human, Mouse
ポジティブサンプル:Jurkat
免疫原:A synthetic phosphorylated peptide around T346 of human NDRG1 (NP_001128714.1).
精製方法:Affinity purification
ストレージバッファ:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:SHTS E
遺伝子ID:10397
Uniprot:Q92597
セルラーロケーション:Cell membrane, Cytoplasm, Nucleus, centrosome, cytoskeleton, cytosol, microtubule organizing center
計算された分子量:33kDa/35kDa/42kDa
観察された分子量:50kDa
Anti-Phospho-NDRG1-T346 pAb (CABP0792)Western blot analysis of extracts of Jurkat cells, using Phospho-NDRG1-T346 antibody (CABP0792) at 1:2000 dilution. Jurkat cells were treated by Calyculin A (100nM) for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
同義語:NDRG1, CAP43, CMT4D, DRG-1, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5
バックグラウンド:This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
UniProt Protein Function:NDRG1: a hypoxia-inducible anti-apoptotic protein. Promotes proliferation and correlates with invasion, metastasis and poor prognosis in many cancers. Is involved in growth arrest and cell differentiation during development. A ubiquitous Rab4a effector protein that modulates angiogenesis and is involved in vesicular recycling of E-cadherin. Colocalizes with transferrin during the recycling in cells. NDRG1 knockdown delays the recycling rate of transferrin, while its overexpression increases the rate of transferrin recycling. Interacts with SIRT1/p53 signaling to attenuate hypoxic injury in human trophoblasts. Its expression is induced by an elevation of free intracellular calcium ion caused by nickel ion exposure. Highly expressed in placental membranes, prostate, kidney, small intestine, ovary, peripheral nerves and Schwann cells. Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D).
UniProt Protein Details:

Protein type:Cell development/differentiation; Vesicle

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: microtubule cytoskeleton; microtubule; centrosome; cell-cell adherens junction; recycling endosome membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function:protein binding; gamma-tubulin binding; cadherin binding; microtubule binding; Rab GTPase binding

Biological Process: response to metal ion; DNA damage response, signal transduction by p53 class mediator; myelin maintenance in the peripheral nervous system; mast cell activation

Disease: Charcot-marie-tooth Disease, Type 4d

NCBI Summary:This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
UniProt Code:Q92597
NCBI GenInfo Identifier:6166568
NCBI Gene ID:10397
NCBI Accession:Q92597.1
UniProt Secondary Accession:Q92597,O15207, Q6IBG2, Q9NYR6, Q9UK29, B3KR80, B7Z446
UniProt Related Accession:Q92597
Molecular Weight:394
NCBI Full Name:Protein NDRG1
NCBI Synonym Full Names:N-myc downstream regulated 1
NCBI Official Symbol:NDRG1  
NCBI Official Synonym Symbols:GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1  
NCBI Protein Information:protein NDRG1; protein regulated by oxygen-1; differentiation-related gene 1 protein; nickel-specific induction protein Cap43; N-myc downstream-regulated gene 1 protein; reducing agents and tunicamycin-responsive protein
UniProt Protein Name:Protein NDRG1
UniProt Synonym Protein Names:Differentiation-related gene 1 protein; DRG-1; N-myc downstream-regulated gene 1 protein; Nickel-specific induction protein Cap43; Reducing agents and tunicamycin-responsive protein; RTP; Rit42
Protein Family:Protein
UniProt Gene Name:NDRG1  
UniProt Entry Name:NDRG1_HUMAN
View AllClose

View AllClose

Related Products