Anti-Phospho-LIM domain kinase 2-T505 Antibody (CABP0388)
- SKU:
- CABP0388
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
抗体名: | Anti-Phospho-LIM domain kinase 2-T505 Antibody |
抗体コード: | CABP0388 |
抗体サイズ: | 50uL, 100uL |
申し込み: | WB IHC IF |
反応性: | Human, Mouse, Rat |
宿主種: | Rabbit |
免疫原: | A phospho specific peptide corresponding to residues surrounding T505 of human LIMK2 |
申し込み: | WB IHC IF |
推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:100 - 1:200 |
反応性: | Human, Mouse, Rat |
ポジティブサンプル: | HeLa |
免疫原: | A phospho specific peptide corresponding to residues surrounding T505 of human LIMK2 |
精製方法: | Affinity purification |
ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
アイソタイプ: | IgG |
順序: | Email for sequence |
遺伝子ID: | 3985 |
Uniprot: | P53671 |
セルラーロケーション: | Cytoplasm, Nucleus, Nucleus |
計算された分子量: | 69kDa/72kDa/77kDa |
観察された分子量: | 72kDa |
同義語: | LIMK2 |
バックグラウンド: | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. |
UniProt Protein Function: | LIMK2: a TKL kinase of the LISK family that regulates actin dynamics. Contains two N-terminal LIM motifs. Phosphorylated and activated by PAK1 and ROCK, downstream effectors of Rho. Phosphorylates cofilin, inhibiting its actin-depolymerizing activity. May contributes to Rho-induced reorganization of the actin cytoskeleton. Two alternative splice variants have been identified. |
UniProt Protein Details: | Protein type:Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; TKL group; LISK family; LIMK subfamily Chromosomal Location of Human Ortholog: 22q12.2 Cellular Component: cis-Golgi network; cytoplasm; nucleus Molecular Function:protein serine/threonine kinase activity; protein binding; zinc ion binding; protein heterodimerization activity; ATP binding Biological Process: spermatogenesis; phosphorylation; protein amino acid phosphorylation |
NCBI Summary: | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
UniProt Code: | P53671 |
NCBI GenInfo Identifier: | 1708824 |
NCBI Gene ID: | 3985 |
NCBI Accession: | P53671.1 |
UniProt Secondary Accession: | P53671,Q7KZ80, Q7L3H5, Q96E10, Q99464, Q9UFU0, A8K6H5 |
UniProt Related Accession: | P53671 |
Molecular Weight: | 638 |
NCBI Full Name: | LIM domain kinase 2 |
NCBI Synonym Full Names: | LIM domain kinase 2 |
NCBI Official Symbol: | LIMK2 |
NCBI Protein Information: | LIM domain kinase 2 |
UniProt Protein Name: | LIM domain kinase 2 |
Protein Family: | LIM domain kinase |
UniProt Gene Name: | LIMK2 |
UniProt Entry Name: | LIMK2_HUMAN |