Anti-PAK2 Antibody (RACO0414)
- SKU:
- RACO0414
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- ELISA
- Application:
- FC
- Conjugation:
- Unconjugated
Description
商品名: | PAK2 Antibody |
Product SKU: | RACO0414 |
サイズ: | 50ul |
宿主種: | Homo sapiens (Human) |
申し込み: | ELISA, WB, FC, IP |
推奨される希釈: | WB:1:500-1:5000, FC:1:20-1:200, IP:1:200-1:1000 |
反応性: | Human, Mouse, Rat |
免疫原: | A synthesized peptide derived from human PAK2 |
憲法: | Liquid |
ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
精製方法: | Affinity-chromatography |
抗体のクローン性: | Monoclonal |
アイソタイプ: | Rabbit IgG |
Conjugate: | Non-conjugated |
バックグラウンド: | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation. |
シノニム: | Serine/threonine-protein kinase PAK 2 (EC 2.7.11.1) (Gamma-PAK) (PAK65) (S6/H4 kinase) (p21-activated kinase 2) (PAK-2) (p58) [Cleaved into: PAK-2p27 (p27), PAK-2p34 (p34) (C-t-PAK2)], PAK2 |
Overlay histogram showing HepG2 cells stained with RACO0414 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg) 1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg) 1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. | |
Immunoprecipitating PAK2 in Raji whole cell lysate Lane 1: Rabbit control IgG instead of RACO0414 in Raji whole cell lysate For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: RACO0414(2µg)+ Raji whole cell lysate (500µg). Lane 3: Raji whole cell lysate (10µg). | |
Western Blot. Positive WB detected in 293 whole cell lysate Jurkat whole cell lysate Raji whole cell lysate Mouse brain tissue, Rat brain tissue. All lanes PAK2 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 59 kDa. Observed band size: 59 kDa. |
UniProt Protein Function: | PAK2: a protein kinase of the STE20 family. Critical effector linking RhoGTPases to cytoskeleton reorganization and nuclear signaling. Activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. |
UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, STE; Kinase, protein; STE group; STE20 family; PAKA subfamily Chromosomal Location of Human Ortholog: 3q29 Cellular Component: cytoplasm; cytosol; nucleoplasm; perinuclear region of cytoplasm; plasma membrane Molecular Function:ATP binding; identical protein binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; protein tyrosine kinase activator activity; Rac GTPase binding Biological Process: actin cytoskeleton organization and biogenesis; apoptosis; axon guidance; cell migration; cell structure disassembly during apoptosis; innate immune response; negative regulation of apoptosis; negative regulation of protein kinase activity; peptidyl-serine phosphorylation; phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; programmed cell death; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of defense response to virus by virus; regulation of growth; regulation of MAPKKK cascade; regulation of mitotic cell cycle; Rho protein signal transduction; signal transduction; small GTPase mediated signal transduction; stimulatory C-type lectin receptor signaling pathway; stress-activated protein kinase signaling pathway; T cell costimulation; T cell receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; viral reproduction |
NCBI Summary: | The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q13177 |
NCBI GenInfo Identifier: | 143811432 |
NCBI Gene ID: | 5062 |
NCBI Accession: | Q13177.3 |
UniProt Secondary Accession: | Q13177,Q13154, Q6ISC3, |
UniProt Related Accession: | Q13177 |
Molecular Weight: | |
NCBI Full Name: | Serine/threonine-protein kinase PAK 2 |
NCBI Synonym Full Names: | p21 protein (Cdc42/Rac)-activated kinase 2 |
NCBI Official Symbol: | PAK2 |
NCBI Official Synonym Symbols: | PAK65; PAKgamma |
NCBI Protein Information: | serine/threonine-protein kinase PAK 2 |
UniProt Protein Name: | Serine/threonine-protein kinase PAK 2 |
UniProt Synonym Protein Names: | Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2 |
Protein Family: | Serine/threonine-protein kinase |
UniProt Gene Name: | PAK2 |
UniProt Entry Name: | PAK2_HUMAN |