Anti-NEDD4 Antibody (CAB0552)
- SKU:
- CAB0552
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
抗体名: | Anti-NEDD4 Antibody |
抗体コード: | CAB0552 |
抗体サイズ: | 20uL, 50uL, 100uL |
申し込み: | WB IHC |
反応性: | Human |
宿主種: | Rabbit |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-460 of human NEDD4 (NP_940682.2). |
申し込み: | WB IHC |
推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
反応性: | Human |
ポジティブサンプル: | U-251MG, HeLa, Jurkat, A-549, SKOV3 |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-460 of human NEDD4 (NP_940682.2). |
精製方法: | Affinity purification |
ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
アイソタイプ: | IgG |
順序: | GSYS SNGS DFGS CASI TSGG SYTN SVIS DSSS YTFP PSDD TFLG GNLP SDST SNRS VPNR NTTP CEIF SRST STDP FVQD DLEH GLEI MKLP VSRN TKIP LKRY SSLV IFPR SPST TRPT SPTS LCTL LSKG SYQT SHQF IISP SEIA HNED GTSA KGFL STAV NGLR LSKT ICTP GEVR DIRP LHRK GSLQ KKIV LSNN TPRQ TVCE KSSE GYSC VSVH FTQR KAAT LDCE TTNG DCKP EMSE IKLN SDSE YIKL MHRT SACL PSSQ NVDC QINI NGEL ERPH SQMN KNHG ILRR SISL G |
遺伝子ID: | 4734 |
Uniprot: | P46934 |
セルラーロケーション: | Cell membrane, Cytoplasm, Peripheral membrane protein |
計算された分子量: | 104kDa/140kDa/147kDa/149kDa |
観察された分子量: | 120kDa |
同義語: | NEDD4, NEDD4-1, RPF1 |
バックグラウンド: | This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. |
UniProt Protein Function: | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins (PubMed:23644597). Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046). |
UniProt Code: | P46934 |
NCBI GenInfo Identifier: | 313104311 |
NCBI Gene ID: | 4734 |
NCBI Accession: | P46934.4 |
UniProt Secondary Accession: | P46934,A1KY35, A6ND72, A7MD29, B4E2R7, B7ZM59, B7ZM60 B9EGN5, D6RF89, |
UniProt Related Accession: | P46934 |
Molecular Weight: | 1319 |
NCBI Full Name: | E3 ubiquitin-protein ligase NEDD4 |
NCBI Synonym Full Names: | neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase |
NCBI Official Symbol: | NEDD4 |
NCBI Official Synonym Symbols: | RPF1; NEDD4-1 |
NCBI Protein Information: | E3 ubiquitin-protein ligase NEDD4; receptor-potentiating factor 1; cell proliferation-inducing gene 53 protein; neural precursor cell expressed developmentally down-regulated protein 4 |
UniProt Protein Name: | E3 ubiquitin-protein ligase NEDD4 |
UniProt Synonym Protein Names: | Cell proliferation-inducing gene 53 protein; Neural precursor cell expressed developmentally down-regulated protein 4; NEDD-4 |
Protein Family: | Nedd4 binding protein |
UniProt Gene Name: | NEDD4 |
UniProt Entry Name: | NEDD4_HUMAN |
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