Anti-Myc-Tag Antibody (CABE070)

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SKU:
CABE070
Product type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
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Description

system_update_altデータシートsystem_update_alt安全性データシート
抗体名:Anti-Myc-Tag Antibody
抗体コード:CABE070
抗体サイズ:100 uL
申し込み:WB IF IP
反応性:
宿主種:Rabbit
免疫原:A synthetic peptide corresponding to Myc tag.
申し込み:WB IF IP
推奨希釈:WB 1:5000-1:20000 IF 1:50 - 1:200 IP 1:500-1:1000
反応性:
ポジティブサンプル:293T
免疫原:A synthetic peptide corresponding to Myc tag.
精製方法:Affinity purification
ストレージバッファ:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:EQKL ISEE DL
遺伝子ID:
Uniprot:
セルラーロケーション:
計算された分子量:
観察された分子量:Refer to Figures
Anti-Myc-Tag AntibodyWestern blot analysis of extracts of normal 293T cells and 293T transfected with Myc-PTEN protein, using Myc-Tag antibody (CABE070) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-Myc-Tag AntibodyImmunofluorescence analysis of HeLa cells transfected with Myc-Tag and untreated HeLa cells use Rabbit anti Myc-Tag mAb(CABE070) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-Myc-Tag AntibodyImmunoprecipitation analysis of 200ug extracts of 293T cells using 3ug Rabbit anti Myc-Tag mAb (CABE070). Western blot was performed from the immunoprecipitate using Rabbit anti Myc-Tag mAb (CABE070) at a dilition of 1:1000.
同義語:Myc, Myc tag, Myc-tag
バックグラウンド:Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
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