Anti-IRF3 抗体 (CAB11118)

抗体名:	Anti-IRF3 Antibody [KO Validated]
抗体コード:	CAB11118
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IP ChIP
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human IRF3 (NP_001184051.1).

申し込み:	WB IHC IP ChIP
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 ChIP 1:20 - 1:100
反応性:	Human, Mouse, Rat
ポジティブサンプル:	293T, HeLa, Jurkat, HepG2

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human IRF3 (NP_001184051.1).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	MGTP KPRI LPWL VSQL DLGQ LEGV AWVN KSRT RFRI PWKH GLRQ DAQQ EDFG IFQA WAEA TGAY VPGR DKPD LPTW KRNF RSAL NRKE GLRL AEDR SKDP HDPH KIYE FVNS GVGD FSQP DTSP DTNG GGST SDTQ EDIL DELL GNMV LAPL PDPG PPSL AVAP EPCP QPLR SPSL DNPT PFPN LGPS ENPL KRLL VPGE EWEF EVTA FYRG RQVF QQTI SCPE GLRL VGSE VGDR TLPG WPVT LPDP GMSL TDRG VMSY
遺伝子ID:	3661
Uniprot:	Q14653
セルラーロケーション:	Cytoplasm, Nucleus
計算された分子量:	12kDa/16kDa/33kDa/47kDa/49kDa
観察された分子量:	56kDa

同義語:

IIAE7, IRF3

バックグラウンド:

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	IRF3: interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 19q13.3-q13.4 Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol Molecular Function:identical protein binding; protein binding; protein homodimerization activity; DNA binding; transcription cofactor activity; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; negative regulation of interferon-beta biosynthetic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; apoptosis; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of defense response to virus by host; response to exogenous dsRNA; positive regulation of interferon type I production; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; positive regulation of interferon-beta production; innate immune response; interferon type I biosynthetic process; defense response to virus; toll-like receptor 4 signaling pathway; response to DNA damage stimulus; negative regulation of interferon type I production; positive regulation of interferon-alpha production; positive regulation of cytokine secretion Disease: Herpes Simplex Encephalitis, Susceptibility To, 7
NCBI Summary:	This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
UniProt Code:	Q14653
NCBI GenInfo Identifier:	2497442
NCBI Gene ID:	3661
NCBI Accession:	Q14653.1
UniProt Secondary Accession:	Q14653,Q5FBY1, Q5FBY2, Q5FBY4, Q7Z5G6, A8K7L2, B2RAZ3
UniProt Related Accession:	Q14653
Molecular Weight:	12,330 Da
NCBI Full Name:	Interferon regulatory factor 3
NCBI Synonym Full Names:	interferon regulatory factor 3
NCBI Official Symbol:	IRF3
NCBI Protein Information:	interferon regulatory factor 3
UniProt Protein Name:	Interferon regulatory factor 3
Protein Family:	Interferon regulatory factor
UniProt Gene Name:	IRF3
UniProt Entry Name:	IRF3_HUMAN

	Western blot analysis of extracts of various cell lines, using IRF3 antibody (CAB11118) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts from normal (control) and [KO Validated] IRF3 Rabbit pAb knockout (KO) HeLa cells, using [KO Validated] IRF3 Rabbit pAb antibody (CAB11118) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human lung cancer using IRF3 antibody (CAB11118) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using IRF3 antibody (CAB11118) at dilution of 1:100 (40x lens).
	Chromatin immunoprecipitation analysis of extracts of HCT116 cell line, using IRF3 rabbit polyclonal antibody (CAB11118) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Antibodies	ELISA Kits
Anti-IRF3 Antibody (CAB0816)[KO Validated]	Human IRF3 ELISA Kit
Anti-IRF3 Antibody (CAB11373)	Human Interferon regulatory factor 3 (IRF3) ELISA Kit
Anti-IRF3 Antibody (CAB2172)	IRF-3 (Phospho-Ser396) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-IRF3-S396 Antibody (CABP0623)	IRF-3 (Phospho-Ser385) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-IRF3-S386 pAb Antibody (CABP0857)	IRF-3 (Phospho-Ser386) Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)