Anti-HPRT1 Antibody (CAB5692)[KO Validated]
- SKU:
- CAB5692
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
抗体名: | Anti-HPRT1 Antibody [KO Validated] |
抗体コード: | CAB5692 |
抗体サイズ: | 20uL, 50uL, 100uL |
申し込み: | WB IF |
反応性: | Human, Mouse, Rat |
宿主種: | Rabbit |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human HPRT1 (NP_000185.1). |
申し込み: | WB IF |
推奨希釈: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
反応性: | Human, Mouse, Rat |
ポジティブサンプル: | MCF-7, HL-60, SW480, 293T, HeLa, Mouse brain, Mouse liver |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human HPRT1 (NP_000185.1). |
精製方法: | Affinity purification |
ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
アイソタイプ: | IgG |
順序: | MATR SPGV VISD DEPG YDLD LFCI PNHY AEDL ERVF IPHG LIMD RTER LARD VMKE MGGH HIVA LCVL KGGY KFFA DLLD YIKA LNRN SDRS IPMT VDFI RLKS YCND QSTG DIKV IGGD DLST LTGK NVLI VEDI IDTG KTMQ TLLS LVRQ YNPK MVKV ASLL VKRT PRSV GYKP DFVG FEIP DKFV VGYA LDYN EYFR DLNH VCVI SETG KAKY KA |
遺伝子ID: | 3251 |
Uniprot: | P00492 |
セルラーロケーション: | Cytoplasm |
計算された分子量: | 24kDa |
観察された分子量: | 27kDa |
同義語: | HPRT1, HGPRT, HPRT |
バックグラウンド: | The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout. |
UniProt Protein Function: | HPRT1: Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5- phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. Defects in HPRT1 are the cause of Lesch-Nyhan syndrome (LNS). LNS is characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation. Defects in HPRT1 are the cause of gout HPRT-related (GOUT-HPRT); also known as HPRT-related gout or Kelley-Seegmiller syndrome. Gout is characterized by partial enzyme activity and hyperuricemia. Belongs to the purine/pyrimidine phosphoribosyltransferase family. |
UniProt Protein Details: | Protein type:EC 2.4.2.8; Xenobiotic Metabolism - drug metabolism - other enzymes; Transferase; Nucleotide Metabolism - purine; Cell development/differentiation Chromosomal Location of Human Ortholog: Xq26.1 Cellular Component: cytoplasm; cytosol Molecular Function:protein binding; protein homodimerization activity; hypoxanthine phosphoribosyltransferase activity; magnesium ion binding; nucleotide binding Biological Process: grooming behavior; lymphocyte proliferation; hypoxanthine metabolic process; IMP salvage; striatum development; nucleobase, nucleoside and nucleotide metabolic process; GMP salvage; dendrite morphogenesis; cytolysis; response to amphetamine; locomotory behavior; adenine salvage; purine nucleotide biosynthetic process; dopamine metabolic process; purine base metabolic process; purine salvage; cerebral cortex neuron differentiation; protein homotetramerization; positive regulation of dopamine metabolic process; GMP catabolic process; IMP metabolic process; hypoxanthine salvage; purine ribonucleoside salvage; central nervous system neuron development; guanine salvage Disease: Kelley-seegmiller Syndrome; Lesch-nyhan Syndrome |
NCBI Summary: | The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009] |
UniProt Code: | P00492 |
NCBI GenInfo Identifier: | 123497 |
NCBI Gene ID: | 3251 |
NCBI Accession: | P00492.2 |
UniProt Secondary Accession: | P00492,A6NHF0, B2R8M9, |
UniProt Related Accession: | P00492 |
Molecular Weight: | 218 |
NCBI Full Name: | Hypoxanthine-guanine phosphoribosyltransferase |
NCBI Synonym Full Names: | hypoxanthine phosphoribosyltransferase 1 |
NCBI Official Symbol: | HPRT1 |
NCBI Official Synonym Symbols: | HPRT; HGPRT |
NCBI Protein Information: | hypoxanthine-guanine phosphoribosyltransferase; HGPRTase |
UniProt Protein Name: | Hypoxanthine-guanine phosphoribosyltransferase |
Protein Family: | Hypoxanthine-guanine phosphoribosyltransferase |
UniProt Gene Name: | HPRT1 |
UniProt Entry Name: | HPRT_HUMAN |