Anti-HO-1 Antibody (CAB11102)
- SKU:
- CAB11102
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| 抗体名: | Anti-HO-1 Antibody |
| 抗体コード: | CAB11102 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-288 of human HO-1 (NP_002124.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | A-549, Mouse kidney, Mouse spleen, Rat kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-288 of human HO-1 (NP_002124.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MERP QPDS MPQD LSEA LKEA TKEV HTQA ENAE FMRN FQKG QVTR DGFK LVMA SLYH IYVA LEEE IERN KESP VFAP VYFP EELH RKAA LEQD LAFW YGPR WQEV IPYT PAMQ RYVK RLHE VGRT EPEL LVAH AYTR YLGD LSGG QVLK KIAQ KALD LPSS GEGL AFFT FPNI ASAT KFKQ LYRS RMNS LEMT PAVR QRVI EEAK TAFL LNIQ LFEE LQEL LTHD TKDQ SPSR APGL RQRA SNKV QDSA PVET PRGK PPLN TRSQ APLL RWVL TLSF LVAT VAVG LYAM |
| 遺伝子ID: | 3162 |
| Uniprot: | P09601 |
| セルラーロケーション: | Cytoplasmic side, Endoplasmic reticulum membrane, Microsome, Peripheral membrane protein |
| 計算された分子量: | 32kDa |
| 観察された分子量: | 32kDa |
| 同義語: | HMOX1, HMOX1D, HO-1, HSP32, bK286B10 |
| バックグラウンド: | Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. |
| UniProt Protein Function: | HMOX1: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA. Expressed at higher levels in renal cancer tissue than in normal tissue. Belongs to the heme oxygenase family. |
| UniProt Protein Details: | Protein type:EC 1.14.99.3; Oxidoreductase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: caveola; cytosol; endoplasmic reticulum; endoplasmic reticulum membrane; extracellular space; membrane; nucleolus; nucleus; perinuclear region of cytoplasm Molecular Function:enzyme binding; heme binding; heme oxygenase (decyclizing) activity; metal ion binding; phospholipase D activity; protein binding; protein homodimerization activity; signal transducer activity Biological Process: angiogenesis; cell death; cellular iron ion homeostasis; cellular response to nutrient; DNA damage response, signal transduction resulting in induction of apoptosis; endothelial cell proliferation; erythrocyte homeostasis; excretion; healing during inflammatory response; heme catabolic process; heme oxidation; iron ion homeostasis; negative regulation of DNA binding; negative regulation of leukocyte migration; negative regulation of mast cell cytokine production; negative regulation of mast cell degranulation; negative regulation of neuron apoptosis; negative regulation of smooth muscle cell proliferation; negative regulation of transcription factor activity; porphyrin metabolic process; positive regulation of angiogenesis; positive regulation of chemokine biosynthetic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of smooth muscle cell proliferation; positive regulation of vasodilation; protein homooligomerization; regulation of angiogenesis; regulation of blood pressure; regulation of transcription factor activity; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; response to estrogen stimulus; response to hydrogen peroxide; response to nicotine; response to oxidative stress; small GTPase mediated signal transduction; smooth muscle hyperplasia; transmembrane transport Disease: Heme Oxygenase 1 Deficiency; Pulmonary Disease, Chronic Obstructive |
| NCBI Summary: | Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P09601 |
| NCBI GenInfo Identifier: | 123446 |
| NCBI Gene ID: | 3162 |
| NCBI Accession: | P09601.1 |
| UniProt Related Accession: | P09601 |
| Molecular Weight: | 32,819 Da |
| NCBI Full Name: | Heme oxygenase 1 |
| NCBI Synonym Full Names: | heme oxygenase 1 |
| NCBI Official Symbol: | HMOX1 |
| NCBI Official Synonym Symbols: | HO-1; HSP32; HMOX1D; bK286B10 |
| NCBI Protein Information: | heme oxygenase 1 |
| UniProt Protein Name: | Heme oxygenase 1 |
| UniProt Gene Name: | HMOX1 |
| UniProt Entry Name: | HMOX1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HO-1 antibody (CAB11102) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat lung using HO-1 antibody (CAB11102) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver using HO-1 antibody (CAB11102) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using HO-1 antibody (CAB11102) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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