Anti-Histone H2B type 1-K Antibody (RACO0039)

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SKU:
RACO0039
Product type:
Recombinant Antibody
Reactivity:
Human
Host Species:
Human
Isotype:
IgG
Application:
WB
Application:
ELISA
Application:
IHC
Conjugation:
Unconjugated
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Description

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商品名:Histone H2B type 1-K Antibody
Product SKU:RACO0039
サイズ:50ul
宿主種:Human
申し込み:ELISA, WB, IHC
推奨される希釈:WB:1:5000-1:10000, IHC:1:50-1:500
反応性:Human
免疫原:A synthesized peptide
憲法:Liquid
ストレージバッファ:Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
精製方法:Affinity-chromatography
抗体のクローン性:Monoclonal
アイソタイプ:Rabbit IgG
Conjugate:Non-conjugated
バックグラウンド:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
シノニム:Histone H2B type 1-K, H2B K, HIRA-interacting protein 1, HIST1H2BK, H2BFT, HIRIP1
Product ImageIHC image of RACO0039 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product ImageWestern Blot. Positive WB detected in Mouse brain tissue, Rat liver tissue, Rat lung tissue. All lanes Histone H2B type 1-K antibody at 0.16µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 14 KDa. Observed band size: 14 KDa.
UniProt Protein Function:H2B1K: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Protein type: DNA-binding Chromosomal Location of Human Ortholog: 6p21.33 Cellular Component: nucleoplasm; extracellular space; nucleosome; nucleus Molecular Function: DNA binding; protein heterodimerization activity Biological Process: establishment and/or maintenance of chromatin architecture; defense response to Gram-positive bacterium; innate immune response in mucosa; antibacterial humoral response
UniProt Protein Details:
NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family. The protein encoded is an antimicrobial protein with antibacterial and antifungal activity. Two transcripts that encode the same protein have been identified for this gene, which is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015]
UniProt Code:O60814
NCBI GenInfo Identifier:18105048
NCBI Gene ID:85236
NCBI Accession:NP_542160.1
UniProt Secondary Accession:O60814,P06899, P23527, P33778, P57053, P58876, P62807 Q16778, Q5QNW6, Q8N257
UniProt Related Accession:O60814
Molecular Weight:14kDa
NCBI Full Name:histone H2B type 1-K
NCBI Synonym Full Names:H2B clustered histone 12
NCBI Official Symbol:H2BC12  
NCBI Official Synonym Symbols:H2BK; H2B/S; H2BFT; H2BFAiii; HIST1H2BK  
NCBI Protein Information:histone H2B type 1-K
UniProt Protein Name:Histone H2B type 1-K
UniProt Synonym Protein Names:HIRA-interacting protein 1
Protein Family:Histone
UniProt Gene Name:HIST1H2BK  
UniProt Entry Name:H2B1K_HUMAN
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