Anti-FASN Antibody (RACO0299)
- SKU:
- RACO0299
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IF
- Application:
- ELISA
- Application:
- FC
- Conjugation:
- Unconjugated
Description
商品名: | FASN Antibody |
Product SKU: | RACO0299 |
サイズ: | 50ul |
宿主種: | Homo sapiens (Human) |
申し込み: | ELISA, IF, FC |
推奨される希釈: | IF:1:20-1:200, FC:1:20-1:200 |
反応性: | Human |
免疫原: | A synthesized peptide derived from human FASN |
憲法: | Liquid |
ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
精製方法: | Affinity-chromatography |
抗体のクローン性: | Monoclonal |
アイソタイプ: | Rabbit IgG |
Conjugate: | Non-conjugated |
バックグラウンド: | Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein. |
シノニム: | Fatty acid synthase (EC 2.3.1.85) [Includes: [Acyl-carrier-protein] S-acetyltransferase (EC 2.3.1.38), [Acyl-carrier-protein] S-malonyltransferase (EC 2.3.1.39), 3-oxoacyl-[acyl-carrier-protein] synthase (EC 2.3.1.41), 3-oxoacyl-[acyl-carrier-protein] reductase (EC 1.1.1.100), 3-hydroxyacyl-[acyl-carrier-protein] dehydratase (EC 4.2.1.59), Enoyl-[acyl-carrier-protein] reductase (EC 1.3.1.39), Oleoyl-[acyl-carrier-protein] hydrolase (EC 3.1.2.14)], FASN, FAS |
Overlay histogram showing A549 cells stained with RACO0299 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg) 1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg) 1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. | |
Immunofluorescence staining of Hela Cells with RACO0299 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
UniProt Protein Function: | FASN: a multifunctional enzyme. Catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. |
UniProt Protein Details: | Protein type:EC 1.3.1.39; EC 1.1.1.100; EC 2.3.1.39; EC 3.1.2.14; Hydrolase; EC 2.3.1.41; EC 4.2.1.59; Oxidoreductase; EC 2.3.1.38; Lyase; EC 2.3.1.85; Lipid Metabolism - fatty acid biosynthesis; Acetyltransferase Chromosomal Location of Human Ortholog: 17q25 Cellular Component: Golgi apparatus; membrane; glycogen granule; mitochondrion; cytoplasm; plasma membrane; melanosome; cytosol Molecular Function:fatty-acid synthase activity; protein homodimerization activity; myristoyl-[acyl-carrier-protein] hydrolase activity; 3-oxoacyl-[acyl-carrier-protein] synthase activity; zinc ion binding; enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity; enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity; drug binding; [acyl-carrier-protein] S-acetyltransferase activity; 3-oxoacyl-[acyl-carrier-protein] reductase activity; protein binding; 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; oleoyl-[acyl-carrier-protein] hydrolase activity; palmitoyl-[acyl-carrier-protein] hydrolase activity; [acyl-carrier-protein] S-malonyltransferase activity; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity Biological Process: osteoblast differentiation; vitamin metabolic process; acetyl-CoA metabolic process; pantothenate metabolic process; energy reserve metabolic process; positive regulation of cellular metabolic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; fatty acid metabolic process; fatty acid biosynthetic process; water-soluble vitamin metabolic process |
NCBI Summary: | The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008] |
UniProt Code: | P49327 |
NCBI GenInfo Identifier: | 269849686 |
NCBI Gene ID: | 2194 |
NCBI Accession: | P49327.3 |
UniProt Secondary Accession: | P49327,Q13479, Q16702, Q4LE83, Q6P4U5, Q6SS02, Q969R1 Q96C68, Q96IT0, |
UniProt Related Accession: | P49327 |
Molecular Weight: | 2511 |
NCBI Full Name: | Fatty acid synthase |
NCBI Synonym Full Names: | fatty acid synthase |
NCBI Official Symbol: | FASN |
NCBI Official Synonym Symbols: | FAS; OA-519; SDR27X1 |
NCBI Protein Information: | fatty acid synthase; short chain dehydrogenase/reductase family 27X, member 1 |
UniProt Protein Name: | Fatty acid synthase |
Protein Family: | Fatty acid synthase |
UniProt Gene Name: | FASN |
UniProt Entry Name: | FAS_HUMAN |