Anti-BUB1 Antibody (RACO0437)
- SKU:
- RACO0437
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Conjugation:
- Unconjugated
Description
商品名: | BUB1 Antibody |
Product SKU: | RACO0437 |
サイズ: | 50ul |
宿主種: | Homo sapiens (Human) |
申し込み: | ELISA, WB |
推奨される希釈: | WB:1:500-1:5000 |
反応性: | Human |
免疫原: | A synthesized peptide derived from human Bub1 |
憲法: | Liquid |
ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
精製方法: | Affinity-chromatography |
抗体のクローン性: | Monoclonal |
アイソタイプ: | Rabbit IgG |
Conjugate: | Non-conjugated |
バックグラウンド: | Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis. |
シノニム: | Mitotic checkpoint serine/threonine-protein kinase BUB1 (hBUB1) (EC 2.7.11.1) (BUB1A), BUB1, BUB1L |
Western Blot. Positive WB detected in Hela whole cell lysate K562 whole cell lysate HepG2 whole cell lysate All lanes Bub1 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 123, 116, 120 kDa. Observed band size: 130 kDa. |
UniProt Protein Function: | BUB1: a mitotic-checkpoint protein kinase of the BUB family. Autophosphorylated when the cells enters mitosis. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index. LOF mutations seen in two colon cancer cell lines exhibiting chromosomal instability, in several leukemias and lymphomas, and in a pancreatic cancer cell line. BUB1 functions in mitotic spindle assembly, and may cause chromosomal instability. In mouse BRCA2 knockouts, mutations in BUB1 and BUBR1 are frequently seen in the resulting tumors. BUB1 and BUBR1 are overexpressed, but not mutated, in many gastric cancers. |
UniProt Protein Details: | Protein type:Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; Kinase, protein; EC 2.7.11.1; Other group; BUB family Chromosomal Location of Human Ortholog: 2q14 Cellular Component: nucleoplasm; kinetochore; membrane; cytoplasm; cytosol; condensed nuclear chromosome, pericentric region Molecular Function:protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity Biological Process: mitosis; cell proliferation; viral reproduction; apoptosis; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; regulation of sister chromatid cohesion; regulation of chromosome segregation; mitotic cell cycle; protein amino acid phosphorylation; chromosome segregation |
NCBI Summary: | This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
UniProt Code: | O43683 |
NCBI GenInfo Identifier: | 8134347 |
NCBI Gene ID: | 699 |
NCBI Accession: | O43683.1 |
UniProt Secondary Accession: | O43683,O43430, O43643, O60626, Q53QE4, E9PC26, F5GXI5 |
UniProt Related Accession: | O43683 |
Molecular Weight: | 120,002 Da |
NCBI Full Name: | Mitotic checkpoint serine/threonine-protein kinase BUB1 |
NCBI Synonym Full Names: | BUB1 mitotic checkpoint serine/threonine kinase |
NCBI Official Symbol: | BUB1 |
NCBI Official Synonym Symbols: | BUB1A; BUB1L; hBUB1 |
NCBI Protein Information: | mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase; budding uninhibited by benzimidazoles 1 homolog; BUB1 budding uninhibited by benzimidazoles 1 homolog |
UniProt Protein Name: | Mitotic checkpoint serine/threonine-protein kinase BUB1 |
UniProt Synonym Protein Names: | BUB1A |
Protein Family: | Checkpoint serine/threonine-protein kinase |
UniProt Gene Name: | BUB1 |
UniProt Entry Name: | BUB1_HUMAN |