Anti-BIRC5 Antibody (RACO0497)
- SKU:
- RACO0497
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
商品名: | BIRC5 Antibody |
Product SKU: | RACO0497 |
サイズ: | 50ul |
宿主種: | Homo sapiens (Human) |
申し込み: | ELISA, IHC |
推奨される希釈: | IHC:1:50-1:200 |
反応性: | Human |
免疫原: | A synthesized peptide derived from human Survivin |
憲法: | Liquid |
ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
精製方法: | Affinity-chromatography |
抗体のクローン性: | Monoclonal |
アイソタイプ: | Rabbit IgG |
Conjugate: | Non-conjugated |
バックグラウンド: | Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:9859993, PubMed:21364656, PubMed:20627126). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797). |
シノニム: | Baculoviral IAP repeat-containing protein 5 (Apoptosis inhibitor 4) (Apoptosis inhibitor survivin), BIRC5, API4 IAP4 |
IHC image of RACO0497 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
UniProt Protein Function: | Survivin: an apoptosis inhibitor that is expressed during the G2/M phase of the cell cycle. Associates with the microtubules of the mitotic spindle and any disruption results in the loss of apoptosis activity. May play a role in neoplasia. Inhibitor of caspase-3 and caspase-7. Two splice variant isoforms have been described. |
UniProt Protein Details: | Protein type:Apoptosis Chromosomal Location of Human Ortholog: 17q25 Cellular Component: centriole; nuclear chromosome; microtubule; spindle microtubule; cytoplasmic microtubule; cytoplasm; spindle; midbody; interphase microtubule organizing center; nucleus; cytosol; chromosome, pericentric region Molecular Function:tubulin binding; identical protein binding; protein homodimerization activity; zinc ion binding; microtubule binding; metal ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; protein binding; enzyme binding; protein heterodimerization activity; chaperone binding; cofactor binding; cysteine protease inhibitor activity; Ran GTPase binding; cobalt ion binding Biological Process: mitosis; transcription, DNA-dependent; spindle checkpoint; establishment of chromosome localization; regulation of signal transduction; protein ubiquitination; cytokinesis; negative regulation of caspase activity; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; chromosome segregation; protein complex localization; cell division; positive regulation of cell proliferation; mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; positive regulation of exit from mitosis |
NCBI Summary: | This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011] |
UniProt Code: | O15392 |
NCBI GenInfo Identifier: | 380865472 |
NCBI Gene ID: | 332 |
NCBI Accession: | O15392.3 |
UniProt Secondary Accession: | O15392,Q2I3N8, Q4VGX0, Q53F61, Q5MGC6, Q6FHL2, Q75SP2 Q9P2W8, A2SUH6, B2R4R1, |
UniProt Related Accession: | O15392 |
Molecular Weight: | 8,490 Da |
NCBI Full Name: | Baculoviral IAP repeat-containing protein 5 |
NCBI Synonym Full Names: | baculoviral IAP repeat containing 5 |
NCBI Official Symbol: | BIRC5 |
NCBI Official Synonym Symbols: | API4; EPR-1 |
NCBI Protein Information: | baculoviral IAP repeat-containing protein 5; apoptosis inhibitor 4; survivin variant 3 alpha; apoptosis inhibitor survivin |
UniProt Protein Name: | Baculoviral IAP repeat-containing protein 5 |
UniProt Synonym Protein Names: | Apoptosis inhibitor 4; Apoptosis inhibitor survivin |
Protein Family: | Baculoviral IAP repeat-containing protein |
UniProt Gene Name: | BIRC5 |
UniProt Entry Name: | BIRC5_HUMAN |