Anti-BAG3 Antibody (RACO0206)
- SKU:
- RACO0206
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
商品名: | BAG3 Antibody |
Product SKU: | RACO0206 |
サイズ: | 50ul |
宿主種: | Human |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
反応性: | Human |
免疫原: | A synthesized peptide derived from human BAG3 |
憲法: | Liquid |
ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
精製方法: | Affinity-chromatography |
抗体のクローン性: | Monoclonal |
アイソタイプ: | Rabbit IgG |
Conjugate: | Non-conjugated |
バックグラウンド: | Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed:9873016, PubMed:27474739). Has anti-apoptotic activity (PubMed:10597216). Plays a role in the HSF1 nucleocytoplasmic transport (PubMed:26159920). |
シノニム: | BAG family molecular chaperone regulator 3, BAG-3, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, Docking protein CAIR-1, BAG3, BIS |
IHC image of RACO0206 diluted at 1:73.75 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Western Blot. Positive WB detected in Hela whole cell lysate 293 whole cell lysate A549 whole cell lysate HepG2 whole cell lysate U87 whole cell lysate All lanes BAG3 antibody at 0. (3µg) ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 62 KDa. Observed band size: 80 KDa. |
UniProt Protein Function: | BAG3: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. Defects in BAG3 are the cause of myopathy myofibrillar type 6 (MFM6). A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material. Defects in BAG3 are the cause of cardiomyopathy dilated type 1HH (CMD1HH). CMD1HH is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. |
UniProt Protein Details: | Protein type:Apoptosis Chromosomal Location of Human Ortholog: 10q26.11 Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; plasma membrane Molecular Function:adenyl-nucleotide exchange factor activity; protein binding Biological Process: induction of apoptosis via death domain receptors Disease: Cardiomyopathy, Dilated, 1hh; Myopathy, Myofibrillar, 6 |
NCBI Summary: | BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008] |
UniProt Code: | O95817 |
NCBI GenInfo Identifier: | 12643665 |
NCBI Gene ID: | 9531 |
NCBI Accession: | O95817.3 |
UniProt Secondary Accession: | O95817,Q3B763, Q9NT20, Q9P120, A8K5L8, |
UniProt Related Accession: | O95817 |
Molecular Weight: | 61,595 Da |
NCBI Full Name: | BAG family molecular chaperone regulator 3 |
NCBI Synonym Full Names: | BCL2 associated athanogene 3 |
NCBI Official Symbol: | BAG3 |
NCBI Official Synonym Symbols: | BIS; MFM6; BAG-3; CAIR-1 |
NCBI Protein Information: | BAG family molecular chaperone regulator 3 |
UniProt Protein Name: | BAG family molecular chaperone regulator 3 |
UniProt Synonym Protein Names: | Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; Docking protein CAIR-1 |
Protein Family: | BAG family molecular chaperone regulator |
UniProt Gene Name: | BAG3 |