Anti-APOA2 Antibody (RACO0199)
- SKU:
- RACO0199
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Application:
- FC
- Conjugation:
- Unconjugated
Description
商品名: | APOA2 Antibody |
Product SKU: | RACO0199 |
サイズ: | 50ul |
宿主種: | Human |
申し込み: | ELISA, WB, IHC, FC |
推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
反応性: | Human |
免疫原: | A synthesized peptide derived from human APOA2 |
憲法: | Liquid |
ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
精製方法: | Affinity-chromatography |
抗体のクローン性: | Monoclonal |
アイソタイプ: | Rabbit IgG |
Conjugate: | Non-conjugated |
バックグラウンド: | May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. |
シノニム: | Apolipoprotein A-II, Apo-AII, ApoA-II, Apolipoprotein A2, Proapolipoprotein A-II, ProapoA-II, Truncated apolipoprotein A-II, Apolipoprotein A-II(1-76), APOA2 |
IHC image of RACO0199 diluted at 1:87.5 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Overlay histogram showing A549 cells stained with RACO0199 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. | |
Western Blot. Positive WB detected in 293T whole cell lysate A549 whole cell lysate All lanes Apolipoprotein A II antibody at 0.87µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 12 KDa. Observed band size: 12 KDa. |
UniProt Protein Function: | Function: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. |
UniProt Protein Details: | Subunit structure: Homodimer; disulfide-linked. Also forms a disulfide-linked heterodimer with APOD. Interacts with HCV core protein. Interacts with APOA1BP. Ref.14 Ref.15 Ref.18 Subcellular location: Secreted. Tissue specificity: Plasma; synthesized in the liver and intestine. Post-translational modification: Phosphorylation sites are present in the extracelllular medium. Sequence similarities: Belongs to the apolipoprotein A2 family. Mass spectrometry: Molecular mass is 8701.2 Da from positions 24 - 100. Determined by MALDI. Ref.16Molecular mass is 8823.4 Da from positions 24 - 100. Determined by MALDI. Cysteinylated ApoA-II. Ref.16Molecular mass is 17421.3 Da from positions 24 - 100. Determined by MALDI. Homodimer. Ref.16Molecular mass is 17293.4 Da from positions 24 - 100. Determined by MALDI. Heterodimer with apolipoprotein A-II(1-76). Ref.16Molecular mass is 8578.3 Da from positions 24 - 99. Determined by MALDI. Ref.16Molecular mass is 17166.2 Da from positions 24 - 99. Determined by MALDI. Homodimer. Ref.16 |
NCBI Summary: | This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia. [provided by RefSeq] |
UniProt Code: | P02652 |
NCBI GenInfo Identifier: | 114000 |
NCBI Gene ID: | 336 |
NCBI Accession: | P02652.1 |
UniProt Secondary Accession: | P02652,B2R524, |
UniProt Related Accession: | P02652,Q76EI7 |
Molecular Weight: | 11,175 Da |
NCBI Full Name: | Apolipoprotein A-II |
NCBI Synonym Full Names: | apolipoprotein A-II |
NCBI Official Symbol: | APOA2 |
NCBI Official Synonym Symbols: | apoAII; Apo-AII; ApoA-II |
NCBI Protein Information: | apolipoprotein A-II; apolipoprotein A2; OTTHUMP00000032244 |
UniProt Protein Name: | Apolipoprotein A-II |
UniProt Synonym Protein Names: | Apolipoprotein A2 |
Protein Family: | Apolipoprotein |
UniProt Gene Name: | APOA2 |
UniProt Entry Name: | APOA2_HUMAN |