ALOX12 Antibody (PACO58685)
- SKU:
- PACO58685
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | ALOX12 Antibody (PACO58685) |
抗体コード: | PACO58685 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Arachidonate 12-lipoxygenase, 12S-type protein (508-658AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: ALOX12 antibody at 3.53µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 76 kDa. Observed band size: 76 kDa. | |
IHC image of PACO58685 diluted at 1:500 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO58685 diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acid, generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid, to (12S)-hydroperoxyeicosatetraenoic acid, (12S)-HPETE but can also metabolize linoleic acid, Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation. |
シノニム: | Arachidonate 12-lipoxygenase, 12S-type (12S-LOX) (12S-lipoxygenase) (EC 1.13.11.31) (Lipoxin synthase 12-LO) (EC 3.3.2) (Platelet-type lipoxygenase 12), ALOX12, 12LO LOG12 |
UniProt Protein Function: | ALOX12: Oxygenase and 14,15-leukotriene A4 synthase activity. Belongs to the lipoxygenase family. |
UniProt Protein Details: | Protein type:EC 1.13.11.31; Lipid Metabolism - arachidonic acid; Oxidoreductase; Apoptosis Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: cytoplasm; cytosol; membrane; sarcolemma Molecular Function:arachidonate 12-lipoxygenase activity; hepoxilin A3 synthase activity; hepoxilin-epoxide hydrolase activity; lipoxygenase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding Biological Process: arachidonic acid metabolic process; cell motility; fatty acid oxidation; hepoxilin biosynthetic process; hepoxilin metabolic process; linoleic acid metabolic process; lipoxygenase pathway; negative regulation of apoptosis; positive regulation of angiogenesis; positive regulation of cell adhesion; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation |
UniProt Code: | P18054 |
NCBI GenInfo Identifier: | 125987838 |
NCBI Gene ID: | 239 |
NCBI Accession: | P18054.4 |
UniProt Secondary Accession: | P18054,O95569, Q6ISF8, Q9UQM4, |
UniProt Related Accession: | P18054 |
Molecular Weight: | 75,694 Da |
NCBI Full Name: | Arachidonate 12-lipoxygenase, 12S-type |
NCBI Synonym Full Names: | arachidonate 12-lipoxygenase, 12S type |
NCBI Official Symbol: | ALOX12 |
NCBI Official Synonym Symbols: | LOG12; 12-LOX; 12S-LOX |
NCBI Protein Information: | arachidonate 12-lipoxygenase, 12S-type |
UniProt Protein Name: | Arachidonate 12-lipoxygenase, 12S-type |
UniProt Synonym Protein Names: | Lipoxin synthase 12-LO (EC:3.3.2.-); Platelet-type lipoxygenase 12 |
Protein Family: | Arachidonate 12-lipoxygenase |
UniProt Gene Name: | ALOX12 |
UniProt Entry Name: | LOX12_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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