ADAM15 Antibody (PACO15305)
- SKU:
- PACO15305
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
抗体名: | ADAM15 Antibody (PACO15305) |
抗体コード: | PACO15305 |
サイズ: | 50ul |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC |
推奨される希釈: | ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100 |
反応性: | Human, Mouse, Rat |
免疫原: | Fusion protein of human ADAM15 |
憲法: | Liquid |
ストレージバッファ: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
精製方法: | Antigen affinity purification |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO15305(ADAM15 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200). | |
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Human liver cancer tissue, NIH/3T3 cells, Jurkat cells, Primary antibody: PACO15305(ADAM15 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes. | |
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO15305(ADAM15 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200). |
バックグラウンド: | The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin β chain, β 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. |
シノニム: | ADAM metallopeptidase domain 15 |
UniProt Protein Function: | ADAM15: Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. 11 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis; EC 3.4.24.-; Protease Chromosomal Location of Human Ortholog: 1q21.3 Cellular Component: cell projection; adherens junction; cell surface; acrosome; integral to membrane; plasma membrane Molecular Function:integrin binding; protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; SH3 domain binding Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; apoptosis; cell-matrix adhesion; proteolysis; negative regulation of cell-matrix adhesion; collagen catabolic process; extracellular matrix disassembly; tissue regeneration; innate immune response; negative regulation of cell growth; angiogenesis; negative regulation of cell migration |
NCBI Summary: | The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq] |
UniProt Code: | Q13444 |
NCBI GenInfo Identifier: | 55960136 |
NCBI Gene ID: | 8751 |
NCBI Accession: | |
UniProt Secondary Accession: | Q13444,Q13493, Q53XQ0, Q5SR68, Q5SR69, Q6R267, Q71S61 Q71S62, Q71S63, Q71S64, Q71S65, B3KQU5, |
UniProt Related Accession: | Q13444,Q59GF2 |
Molecular Weight: | 92,959 Da |
NCBI Full Name: | ADAM metallopeptidase domain 15 |
NCBI Synonym Full Names: | ADAM metallopeptidase domain 15 |
NCBI Official Symbol: | ADAM15 |
NCBI Official Synonym Symbols: | MDC15; ADAM15 |
NCBI Protein Information: | disintegrin and metalloproteinase domain-containing protein 15; MDC-15; ADAM 15; metargidin; OTTHUMP00000033236; metalloprotease RGD disintegrin protein; a disintegrin and metalloproteinase domain 15 (metargidin); metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15 |
UniProt Protein Name: | Disintegrin and metalloproteinase domain-containing protein 15 |
UniProt Synonym Protein Names: | Metalloprotease RGD disintegrin protein; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15; MDC-15; Metargidin |
Protein Family: | Disintegrin and metalloproteinase domain-containing protein |
UniProt Gene Name: | ADAM15 |
UniProt Entry Name: | ADA15_HUMAN |