ACAA2 Antibody (PACO53206)
- SKU:
- PACO53206
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Application:
- IP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | ACAA2 Antibody (PACO53206) |
抗体コード: | PACO53206 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IHC, IF, IP |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:500-1:1000, IF:1:50-1:500, IP:1:200-1:2000, |
反応性: | Human, Mouse, Rat |
免疫原: | Recombinant Human 3-ketoacyl-CoA thiolase, mitochondrial protein (188-257AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat lung tissue, Mouse liver tissue, Mouse lung tissue. All lanes: ACAA2 antibody at 2.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 42 kDa. Observed band size: 42 kDa. | |
Immunofluorescent analysis of HepG2 cells using PACO53206 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO53206 diluted at 1:700 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
バックグラウンド: | Abolishes BNIP3-mediated apoptosis and mitochondrial damage. |
シノニム: | 3-ketoacyl-CoA thiolase, mitochondrial (EC 2.3.1.16) (Acetyl-CoA acyltransferase) (Beta-ketothiolase) (Mitochondrial 3-oxoacyl-CoA thiolase) (T1), ACAA2 |
UniProt Protein Function: | ACAA2: Abolishes BNIP3-mediated apoptosis and mitochondrial damage. Belongs to the thiolase family. |
UniProt Protein Details: | Protein type:Amino Acid Metabolism - valine, leucine and isoleucine degradation; EC 2.3.1.16; Lipid Metabolism - fatty acid; Lipid Metabolism - fatty acid elongation in mitochondria; Transferase Chromosomal Location of Human Ortholog: 18q21.1 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:acetyl-CoA C-acyltransferase activity; protein binding; RNA binding Biological Process: fatty acid beta-oxidation |
NCBI Summary: | The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal. [provided by RefSeq, Jul 2008] |
UniProt Code: | P42765 |
NCBI GenInfo Identifier: | 167614485 |
NCBI Gene ID: | 10449 |
NCBI Accession: | NP_006102.2 |
UniProt Secondary Accession: | P42765,Q9BUT6, |
UniProt Related Accession: | P42765 |
Molecular Weight: | |
NCBI Full Name: | 3-ketoacyl-CoA thiolase, mitochondrial |
NCBI Synonym Full Names: | acetyl-CoA acyltransferase 2 |
NCBI Official Symbol: | ACAA2 |
NCBI Official Synonym Symbols: | DSAEC |
NCBI Protein Information: | 3-ketoacyl-CoA thiolase, mitochondrial |
UniProt Protein Name: | 3-ketoacyl-CoA thiolase, mitochondrial |
UniProt Synonym Protein Names: | Acetyl-CoA acyltransferase; Beta-ketothiolase; Mitochondrial 3-oxoacyl-CoA thiolase; T1 |
Protein Family: | 3-ketoacyl-CoA thiolase |
UniProt Gene Name: | ACAA2 |