AANAT Antibody (PACO58172)
- SKU:
- PACO58172
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
抗体名: | AANAT Antibody (PACO58172) |
抗体コード: | PACO58172 |
サイズ: | 50ug |
宿主種: | Rabbit |
申し込み: | ELISA, WB, IF |
推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IF:1:50-1:200 |
反応性: | Human |
免疫原: | Recombinant Human Serotonin N-acetyltransferase protein (1-207AA) |
憲法: | Liquid |
ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
精製方法: | >95%, Protein G purified |
抗体のクローン性: | Polyclonal |
アイソタイプ: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: 293 whole cell lysate, 293T whole cell lysate. All lanes: AANAT antibody at 7.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 24, 29 kDa. Observed band size: 29 kDa. | |
Immunofluorescence staining of A549 cells with PACO58172 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
バックグラウンド: | Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin. |
シノニム: | Serotonin N-acetyltransferase (Serotonin acetylase) (EC 2.3.1.87) (Aralkylamine N-acetyltransferase) (AA-NAT), AANAT, SNAT |
UniProt Protein Function: | AA-NAT var1: Catalyzes the N-acetylation of serotonin into N- acetylserotonin. Controls the night/day rhythm of melatonin production in the pineal gland. Has a relative affinity for hydroxylated versus non-hydroxylated arylalkylamines. Belongs to the acetyltransferase family. AANAT subfamily. Monomer. Interacts with YWHAZ; the interaction requires phosphorylation on Thr-31, and modulates the enzymatic activity of AANAT through preventing dephosphorylation and/or proteolysis and stabilizing substrate binding. Subsequently, a second molecule of AANAT can bind, via the phosphorylated Ser-205 site, the other YWHAZ monomer with similar effect. |
UniProt Protein Details: | Protein type:Acetyltransferase; EC 2.3.1.87; Amino Acid Metabolism - tryptophan Chromosomal Location of Human Ortholog: 17q25.1 Cellular Component: perinuclear region of cytoplasm; cytosol Molecular Function:arylamine N-acetyltransferase activity; aralkylamine N-acetyltransferase activity Biological Process: circadian rhythm; N-terminal protein amino acid acetylation; response to light stimulus; melatonin biosynthetic process; response to copper ion; response to zinc ion; indolalkylamine biosynthetic process; response to cytokine stimulus; response to calcium ion; response to corticosterone stimulus; response to insulin stimulus Disease: Delayed Sleep Phase Syndrome, Susceptibility To |
NCBI Summary: | The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
UniProt Code: | Q16613 |
NCBI GenInfo Identifier: | 11387096 |
NCBI Gene ID: | 15 |
NCBI Accession: | Q16613.1 |
UniProt Secondary Accession: | Q16613,Q562F4, A0AVF2, J3KMZ5, |
UniProt Related Accession: | Q16613 |
Molecular Weight: | 28,432 Da |
NCBI Full Name: | Serotonin N-acetyltransferase |
NCBI Synonym Full Names: | aralkylamine N-acetyltransferase |
NCBI Official Symbol: | AANAT |
NCBI Official Synonym Symbols: | DSPS; SNAT |
NCBI Protein Information: | serotonin N-acetyltransferase; serotonin acetylase; arylalkylamine N-acetyltransferase |
UniProt Protein Name: | Serotonin N-acetyltransferase |
UniProt Synonym Protein Names: | Aralkylamine N-acetyltransferase; AA-NAT |
Protein Family: | Serotonin N-acetyltransferase |
UniProt Gene Name: | AANAT |
UniProt Entry Name: | SNAT_HUMAN |
Antibodies | ELISA Kits |
Anti-AANAT Antibody (CAB11850) | Human AANAT / Serotonin N-acetyltransferase ELISA Kit |
Anti-AANAT Antibody (CAB15016) | Human Serotonin N-acetyltransferase (AANAT) ELISA Kit |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |