CACYBP Antibody (PACO55294)
- SKU:
- PACO55294
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Application:
- IP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 抗体名: | CACYBP Antibody (PACO55294) |
| 抗体コード: | PACO55294 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC, IF, IP |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000, |
| 反応性: | Human |
| 免疫原: | Recombinant Human Calcyclin-binding protein (2-228AA) |
| 憲法: | Liquid |
| ストレージバッファ: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| 精製方法: | >95%, Protein G purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HUVEC whole cell lysate. All lanes: CACYBP antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 27, 9, 22 kDa. Observed band size: 27 kDa. |
 | Immunofluorescence staining of U251 cells with PACO55294 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using PACO55294 at dilution of 1:100. |
| バックグラウンド: | May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1). |
| シノニム: | Calcyclin-binding protein (CacyBP) (hCacyBP) (S100A6-binding protein) (Siah-interacting protein), CACYBP, S100A6BP SIP |
| UniProt Protein Function: | CACYBP: a nuclear/cytoplasmic protein involved in calcium-dependent ubiquitination and proteosomal degradation of target proteins. A possible molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1). Interacts with proteins of the S100 family at physiological calcium concentrations. Component of some large E3 complexes and interacts directly with SIAH1, SIAH2 and SKP1A. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated. Two alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 1q24-q25 Cellular Component: nucleoplasm; neuron projection; nuclear envelope lumen; cytoplasm; beta-catenin destruction complex Molecular Function:protein binding; protein homodimerization activity Biological Process: positive regulation of DNA replication; cardiac muscle cell differentiation; aging |
| NCBI Summary: | The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9HB71 |
| NCBI GenInfo Identifier: | 46576651 |
| NCBI Gene ID: | 27101 |
| NCBI Accession: | Q9HB71.2 |
| UniProt Secondary Accession: | Q9HB71,O60666, Q5R370, Q5R371, B2ZWH2, B3KSF1, |
| UniProt Related Accession: | Q9HB71 |
| Molecular Weight: | 21,228 Da |
| NCBI Full Name: | Calcyclin-binding protein |
| NCBI Synonym Full Names: | calcyclin binding protein |
| NCBI Official Symbol: | CACYBP |
| NCBI Official Synonym Symbols: | SIP; GIG5; PNAS-107; S100A6BP |
| NCBI Protein Information: | calcyclin-binding protein; hCacyBP; S100A6-binding protein; Siah-interacting protein (SIP); growth-inhibiting gene 5 protein |
| UniProt Protein Name: | Calcyclin-binding protein |
| UniProt Synonym Protein Names: | S100A6-binding protein; Siah-interacting protein |
| Protein Family: | Calcyclin-binding protein |
| UniProt Gene Name: | CACYBP |
| UniProt Entry Name: | CYBP_HUMAN |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |