Anti-ALKBH1 Antibody (CAB14079)
- SKU:
- CAB14079
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-ALKBH1 Antibody |
| 抗体コード: | CAB14079 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF IP |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 110-389 of human ALKBH1 (NP_006011.2). |
| 申し込み: | WB IF IP |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:20 - 1:50 |
| 反応性: | Human |
| ポジティブサンプル: | 293T |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 110-389 of human ALKBH1 (NP_006011.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | YQWH WVKQ CLKL YSQK PNVC NLDK HMSK EETQ DLWE QSKE FLRY KEAT KRRP RSLL EKLR WVTV GYHY NWDS KKYS ADHY TPFP SDLG FLSE QVAA ACGF EDFR AEAG ILNY YRLD STLG IHVD RSEL DHSK PLLS FSFG QSAI FLLG GLQR DEAP TAMF MHSG DIMI MSGF SRLL NHAV PRVL PNPE GEGL PHCL EAPL PAVL PRDS MVEP CSME DWQV CASY LKTA RVNM TVRQ VLAT DQNF PLEP IEDE KRDI STEG FCHL DDQN SEVK RARI NPDS |
| 遺伝子ID: | 8846 |
| Uniprot: | Q13686 |
| セルラーロケーション: | Mitochondrion, Nucleus |
| 計算された分子量: | 43kDa |
| 観察された分子量: | 44kDa |
| 同義語: | ALKBH1, ABH, ABH1, ALKBH, alkB, hABH |
| バックグラウンド: | This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. |
| UniProt Protein Function: | ALKBH: Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation. Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron. Belongs to the alkB family. |
| UniProt Protein Details: | Protein type:DNA repair, damage; EC 4.2.99.18; EC 1.14.11.33 Chromosomal Location of Human Ortholog: 14q24.3 Cellular Component: mitochondrion Molecular Function:DNA-(apurinic or apyrimidinic site) lyase activity; ferrous iron binding; chemoattractant activity Biological Process: developmental growth; DNA dealkylation; positive chemotaxis; in utero embryonic development; neuron migration; RNA repair; negative regulation of neuron apoptosis; DNA repair; DNA catabolic process, endonucleolytic; neurite development; placenta development |
| NCBI Summary: | This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13686 |
| NCBI GenInfo Identifier: | 12643239 |
| NCBI Gene ID: | 8846 |
| NCBI Accession: | Q13686.2 |
| UniProt Secondary Accession: | Q13686,Q8TAU1, Q9ULA7, |
| UniProt Related Accession: | Q13686 |
| Molecular Weight: | 389 |
| NCBI Full Name: | Alkylated DNA repair protein alkB homolog 1 |
| NCBI Synonym Full Names: | alkB, alkylation repair homolog 1 (E. coli) |
| NCBI Official Symbol: | ALKBH1 |
| NCBI Official Synonym Symbols: | ABH; ABH1; alkB; hABH; ALKBH |
| NCBI Protein Information: | alkylated DNA repair protein alkB homolog 1; DNA lyase ABH1; alkylation repair, alkB homolog; DNA oxidative demethylase ALKBH1; alpha-ketoglutarate-dependent dioxygenase ABH1 |
| UniProt Protein Name: | Alkylated DNA repair protein alkB homolog 1 |
| UniProt Synonym Protein Names: | Alpha-ketoglutarate-dependent dioxygenase ABH1; DNA lyase ABH1 (EC:4.2.99.18); DNA oxidative demethylase ALKBH1 |
| Protein Family: | DNA demethylase |
| UniProt Gene Name: | ALKBH1 |
| UniProt Entry Name: | ALKB1_HUMAN |
 | Western blot analysis of extracts of 293T cells, using ALKBH1 antibody (CAB14079) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunofluorescence analysis of U2OS cells using ALKBH1 antibody (CAB14079). Blue: DAPI for nuclear staining. |
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