Anti-SLC16A1 Antibody (RACO0438)

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SKU:
RACO0438
Product type:
Recombinant Antibody
Reactivity:
Human
Host Species:
Human
Isotype:
IgG
Application:
WB
Application:
ELISA
Application:
IHC
Conjugation:
Unconjugated
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Description

system_update_altデータシートsystem_update_alt安全性データシート
商品名:SLC16A1 Antibody
Product SKU:RACO0438
サイズ:50ul
宿主種:Homo sapiens (Human)
申し込み:ELISA, WB, IHC
推奨される希釈:WB:1:500-1:5000, IHC:1:50-1:200
反応性:Human
免疫原:A synthesized peptide derived from human Monocarboxylic acid transporter 1
憲法:Liquid
ストレージバッファ:Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
精製方法:Affinity-chromatography
抗体のクローン性:Monoclonal
アイソタイプ:Rabbit IgG
Conjugate:Non-conjugated
バックグラウンド:Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.
シノニム:Monocarboxylate transporter 1 (MCT 1) (Solute carrier family 16 member 1), SLC16A1, MCT1
Product ImageIHC image of RACO0438 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Product ImageIHC image of RACO0438 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Product ImageWestern Blot. Positive WB detected in Hela whole cell lysate HepG2 whole cell lysate Jurkat whole cell lysate 293T whole cell lysate K562 whole cell lysate All lanes MCT1 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 54, 47 kDa. Observed band size: 54 kDa.
UniProt Protein Function:MCT1: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Defects in SLC16A1 are the cause of symptomatic deficiency in lactate transport (SDLT); also known as erythrocyte lactate transporter defect. Deficiency of lactate transporter may result in an acidic intracellular environment created by muscle activity with consequent degeneration of muscle and release of myoglobin and creatine kinase. This defect might compromise extreme performance in otherwise healthy individuals. Defects in SLC16A1 are the cause of familial hyperinsulinemic hypoglycemia type 7 (HHF7); also known as exercise-induced hyperinsulinemic hypoglycemia. HHF7 is a dominantly inherited hypoglycemic disorder characterized by inappropriate insulin secretion during anaerobic exercise or on pyruvate load. Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
UniProt Protein Details:

Protein type:Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral; Transporter; Mitochondrial

Chromosomal Location of Human Ortholog: 1p12

Cellular Component: centrosome; membrane; mitochondrion; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function:mevalonate transmembrane transporter activity; protein homodimerization activity; monocarboxylic acid transmembrane transporter activity; symporter activity; lactate transmembrane transporter activity; secondary active monocarboxylate transmembrane transporter activity

Biological Process: response to food; monocarboxylic acid transport; glucose homeostasis; cellular metabolic process; centrosome organization and biogenesis; mevalonate transport; lipid metabolic process; pyruvate metabolic process; blood coagulation; leukocyte migration; transmembrane transport; regulation of insulin secretion; behavioral response to nutrient

Disease: Erythrocyte Lactate Transporter Defect; Monocarboxylate Transporter 1 Deficiency; Hyperinsulinemic Hypoglycemia, Familial, 7

NCBI Summary:The protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2009]
UniProt Code:P53985
NCBI GenInfo Identifier:313104214
NCBI Gene ID:6566
NCBI Accession:P53985.3
UniProt Secondary Accession:P53985,Q49A45, Q5T8R6, Q9NSJ9,
UniProt Related Accession:P53985
Molecular Weight:46,234 Da
NCBI Full Name:Monocarboxylate transporter 1
NCBI Synonym Full Names:solute carrier family 16 (monocarboxylate transporter), member 1
NCBI Official Symbol:SLC16A1  
NCBI Official Synonym Symbols:MCT; HHF7; MCT1  
NCBI Protein Information:monocarboxylate transporter 1; MCT 1; solute carrier family 16 member 1; solute carrier family 16 (monocarboxylic acid transporters), member 1; solute carrier family 16, member 1 (monocarboxylic acid transporter 1)
UniProt Protein Name:Monocarboxylate transporter 1
UniProt Synonym Protein Names:Solute carrier family 16 member 1
Protein Family:Monocarboxylate transporter
UniProt Gene Name:SLC16A1  
UniProt Entry Name:MOT1_HUMAN
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