Anti-Rabbit anti V5-Tag Polyclonal Antibody (CABE071)

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SKU:
CABE071
Product type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
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Description

system_update_altデータシートsystem_update_alt安全性データシート
抗体名:Anti-Rabbit anti V5-Tag Polyclonal Antibody
抗体コード:CABE071
抗体サイズ:100 uL
申し込み:WB
反応性:
宿主種:Rabbit
免疫原:A synthetic peptide corresponding to V5 tag.
申し込み:WB
推奨希釈:WB 1:1000 - 1:2000
反応性:
ポジティブサンプル:293T
免疫原:A synthetic peptide corresponding to V5 tag.
精製方法:Affinity purification
ストレージバッファ:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:GKPI PNPL LGLD ST
遺伝子ID:
Uniprot:
セルラーロケーション:
計算された分子量:
観察された分子量:Refer to Figures
Anti-Rabbit anti V5-Tag Polyclonal AntibodyWestern blot analysis of extracts of normal 293T cells and 293T transfected with V5-Wnt3a3d protein, using V5-Tag antibody (CABE071) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
同義語:V5, V5 tag, V5-tag
バックグラウンド:Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
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