Anti-Phospho-EphA2-Y772 pAb Antibody (CABP0817)

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SKU:
CABP0817
Product type:
Antibody
Application:
WB
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cardiovascular
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Description

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抗体名:Anti-Phospho-EphA2-Y772 Antibody
抗体コード:CABP0817
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB
反応性:Human, Rat
宿主種:Rabbit
免疫原:A synthetic phosphorylated peptide around Y772 of human EphA2 (NP_004422.2).
申し込み:WB
推奨希釈:WB 1:500 - 1:2000
反応性:Human, Rat
ポジティブサンプル:C6
免疫原:A synthetic phosphorylated peptide around Y772 of human EphA2 (NP_004422.2).
精製方法:Affinity purification
ストレージバッファ:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:ATYT T
遺伝子ID:1969
Uniprot:P29317
セルラーロケーション:Cell junction, Cell membrane, Cell projection, Single-pass type I membrane protein, focal adhesion, lamellipodium membrane, ruffle membrane
計算された分子量:54kDa/108kDa
観察された分子量:108kDa
Anti-Phospho-EphA2-Y772 pAb (CABP0817)Western blot analysis of extracts of C6 cells, using Phospho-EphA2-Y772 antibody (CABP0817) at 1:1000 dilution. C6 cells were treated by EphrinA1 (100 ng/mL) for 5 minutes . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
同義語:ARCC2, CTPA, CTPP1, CTRCT6, ECK, EPHA2
バックグラウンド:This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.
UniProt Protein Function:EphA2: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 AND -A5. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Overexpressed in many cancers including aggressive ovarian, cervical and breast carcinomas, and lung cancer. Expression correlates with degree of angiogenesis, metastasis and xenograft tumor growth. Soluble receptor inhibits tumor growth and angiogenesis in mice.
UniProt Protein Details:

Protein type:Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: cell surface; focal adhesion; integral to plasma membrane; intracellular; plasma membrane

Molecular Function:ATP binding; ephrin receptor activity; protein binding; transmembrane receptor protein tyrosine kinase activity

Biological Process: angiogenesis; axial mesoderm formation; axon guidance; bone remodeling; cell adhesion; cell migration; DNA damage response, signal transduction resulting in induction of apoptosis; ephrin receptor signaling pathway; keratinocyte differentiation; mammary gland epithelial cell proliferation; multicellular organismal development; negative regulation of protein kinase B signaling cascade; neural tube development; notochord cell development; notochord formation; osteoblast differentiation; osteoclast differentiation; peptidyl-tyrosine phosphorylation; protein kinase B signaling cascade; regulation of angiogenesis; regulation of blood vessel endothelial cell migration; regulation of cell adhesion mediated by integrin; skeletal development; vasculogenesis; viral reproduction

Disease: Cataract 6, Multiple Types

NCBI Summary:This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]
UniProt Code:P29317
NCBI GenInfo Identifier:229462861
NCBI Gene ID:1969
NCBI Accession:P29317.2
UniProt Secondary Accession:P29317,Q8N3Z2, B5A968,
UniProt Related Accession:P29317
Molecular Weight:
NCBI Full Name:Ephrin type-A receptor 2
NCBI Synonym Full Names:EPH receptor A2
NCBI Official Symbol:EPHA2  
NCBI Official Synonym Symbols:ECK; CTPA; ARCC2; CTPP1; CTRCT6  
NCBI Protein Information:ephrin type-A receptor 2
UniProt Protein Name:Ephrin type-A receptor 2
UniProt Synonym Protein Names:Epithelial cell kinase; Tyrosine-protein kinase receptor ECK
Protein Family:Ephrin type-A receptor
UniProt Gene Name:EPHA2  
UniProt Entry Name:EPHA2_HUMAN
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