Anti-Phospho-ACLY-S455 pAb Antibody (CABP0779)

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SKU:
CABP0779
Product type:
Antibody
Application:
WB
Application:
IHC
Application:
IP
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
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Description

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抗体名:Anti-Phospho-ACLY-S455 Antibody
抗体コード:CABP0779
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB IHC IP
反応性:Human, Mouse, Rat
宿主種:Rabbit
免疫原:A synthetic phosphorylated peptide around S455 of human ACLY (NP_001087.2).
申し込み:WB IHC IP
推奨希釈:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100
反応性:Human, Mouse, Rat
ポジティブサンプル:NIH/3T3
免疫原:A synthetic phosphorylated peptide around S455 of human ACLY (NP_001087.2).
精製方法:Affinity purification
ストレージバッファ:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:TASF S
遺伝子ID:47
Uniprot:P53396
セルラーロケーション:Cytoplasm
計算された分子量:91kDa/119kDa/120kDa
観察された分子量:121kDa
Anti-Phospho-ACLY-S455 pAb (CABP0779)Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ACLY-S455 antibody (CABP0779) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-Phospho-ACLY-S455 pAb (CABP0779)Immunohistochemistry of paraffin-embedded rat pancreas using Phospho-ACLY-S455 antibody (CABP0779) at dilution of 1:100 (40x lens).
Anti-Phospho-ACLY-S455 pAb (CABP0779)Immunohistochemistry of paraffin-embedded mouse brain using Phospho-ACLY-S455 antibody (CABP0779) at dilution of 1:100 (40x lens).
Anti-Phospho-ACLY-S455 pAb (CABP0779)Immunohistochemistry of paraffin-embedded mouse pancreas using Phospho-ACLY-S455 antibody (CABP0779) at dilution of 1:100 (40x lens).
Anti-Phospho-ACLY-S455 pAb (CABP0779)Immunoprecipitation analysis of 200ug extracts of NIH/3T3 cells, using 3 ug Anti-Phospho-ACLY-S455 pAb (CABP0779). Western blot was performed from the immunoprecipitate using Anti-Phospho-ACLY-S455 pAb (CABP0779) at a dilition of 1:1000. NIH/3T3 cells were treated by Insulin (100 nM) at 37'C for 10 minutes after serum-starvation overnight.
同義語:ACLY, ACL, ATPCL, CLATP
バックグラウンド:ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440, 000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
UniProt Protein Function:ACLY: ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Homotetramer. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.3.3.8; Transferase; Lyase; Carbohydrate Metabolism - citrate (TCA) cycle

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; cytosol; membrane; mitochondrion; nucleoplasm; plasma membrane

Molecular Function:ATP binding; ATP citrate synthase activity; cofactor binding; metal ion binding; protein binding

Biological Process: acetyl-CoA biosynthetic process; cellular lipid metabolic process; cholesterol biosynthetic process; citrate metabolic process; energy reserve metabolic process; fatty acid biosynthetic process; lipid biosynthetic process; oxaloacetate metabolic process; positive regulation of cellular metabolic process; triacylglycerol biosynthetic process

NCBI Summary:ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]
UniProt Code:P53396
NCBI GenInfo Identifier:116241237
NCBI Gene ID:47
NCBI Accession:P53396.3
UniProt Secondary Accession:P53396,Q13037, Q9BRL0, B4DIM0, B4E3P0,
UniProt Related Accession:P53396
Molecular Weight:91,099 Da
NCBI Full Name:ATP-citrate synthase
NCBI Synonym Full Names:ATP citrate lyase
NCBI Official Symbol:ACLY  
NCBI Official Synonym Symbols:ACL; ATPCL; CLATP  
NCBI Protein Information:ATP-citrate synthase
UniProt Protein Name:ATP-citrate synthase
UniProt Synonym Protein Names:ATP-citrate (pro-S-)-lyase; ACL; Citrate cleavage enzyme
Protein Family:ATP-citrate synthase
UniProt Gene Name:ACLY  
UniProt Entry Name:ACLY_HUMAN
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