Anti-DCLK1 抗体 (CAB16464)

抗体名:	Anti-DCLK1 Antibody
抗体コード:	CAB16464
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB
反応性:	Human, Mouse
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 630-729 of human DCLK1 (NP_004725.1).

申し込み:	WB
推奨希釈:	WB 1:500 - 1:2000
反応性:	Human, Mouse
ポジティブサンプル:	HepG2, A-549, 22Rv1, Mouse heart

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 630-729 of human DCLK1 (NP_004725.1).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	VDVD QRFS AVQV LEHP WVND DGLP ENEH QLSV AGKI KKHF NTGP KPNS TAAG VSVI ALDH GFTI KRSG SLDY YQQP GMYW IRPP LLIR RGRF SDED ATRM
遺伝子ID:	9201
Uniprot:	O15075
セルラーロケーション:
計算された分子量:	46kDa/47kDa/81kDa/82kDa
観察された分子量:	82kDa

同義語:

DCLK1, CL1, CLICK1, DCAMKL1, DCDC3A, DCLK

バックグラウンド:

This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.

UniProt Protein Function:	Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
NCBI Summary:	This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.[provided by RefSeq, Sep 2010]
UniProt Code:	O15075
NCBI GenInfo Identifier:	6225242
NCBI Gene ID:	9201
NCBI Accession:	O15075.2
UniProt Secondary Accession:	O15075,Q5VZY8, Q5VZZ0, Q5VZZ1, B7Z3E9,
UniProt Related Accession:	O15075
Molecular Weight:	47,681 Da
NCBI Full Name:	Serine/threonine-protein kinase DCLK1
NCBI Synonym Full Names:	doublecortin like kinase 1
NCBI Official Symbol:	DCLK1
NCBI Official Synonym Symbols:	CL1; DCLK; CLICK1; DCDC3A; DCAMKL1
NCBI Protein Information:	serine/threonine-protein kinase DCLK1
UniProt Protein Name:	Serine/threonine-protein kinase DCLK1
UniProt Synonym Protein Names:	Doublecortin domain-containing protein 3A; Doublecortin-like and CAM kinase-like 1; Doublecortin-like kinase 1
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	DCLK1
UniProt Entry Name:	DCLK1_HUMAN

Western blot analysis of extracts of various cell lines, using DCLK1 antibody (CAB16464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.

Antibodies	ELISA Kits
Anti-DCLK1 Antibody (CAB9474)	Human DCLK1 ELISA Kit
Anti-DCAMKL1 Antibody (CAB4372)	Human DCLK1 (Doublecortin Like Kinase 1) CLIA Kit (HUES00262)
DCLK1 Antibody (PACO08758)	Human DCLK1 (Doublecortin Like Kinase 1) ELISA Kit (HUES01585)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)