Anti-CDK2 抗体 (CAB18000)

抗体名:	Anti-CDK2 Antibody [KO Validated]
抗体コード:	CAB18000
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	A synthetic peptide of human CDK2

申し込み:	WB IHC
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
反応性:	Human, Mouse, Rat
ポジティブサンプル:	293T

免疫原:	A synthetic peptide of human CDK2
精製方法:	Affinity purification
ストレージバッファ:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	Email for sequence
遺伝子ID:	1017
Uniprot:	P24941
セルラーロケーション:	Cajal body, Cytoplasm, Endosome, Nucleus, centrosome, cytoskeleton, microtubule organizing center
計算された分子量:	30kDa/33kDa
観察された分子量:	33kDa

Anti-CDK2 Antibody [KO Validated] (CAB18000)

Western blot analysis of extracts from normal (control) and CDK2 knockout (KO) 293T cells, using CDK2 antibody (CAB18000) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

同義語:

CDK2, CDKN2, p33(CDK2)

バックグラウンド:

This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	CDK2: a protein kinase of the CDK family. An important component of the cell cycle machinery. Activity of CDK2 is maximal during S phase and G2. Cdk2/cyclin E kinase activity is important for the G1 to S transition and phosphorylates the Rb protein. In S-phase, active cdk2/cyclin A complexes predominate and phosphorylate E2F, and the active cdk2 complex persists in the nucleus through G2. Part of the Rb pathway disregulated in most tumors. Target of several candidate cancer drugs. However, inhibition does not always prevent cancer cell growth, possibly due to CDK redundancy. Inhibitors: BMS-265246, BMS-265246-01, R-roscovitine (CYC200, CYC202), SU9516, R547, L868276
UniProt Protein Details:	Protein type:Kinase, protein; EC 2.7.11.22; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; CMGC group; CDK family; CDK1 subfamily; CDK/CDK1 subfamily Chromosomal Location of Human Ortholog: 12q13 Cellular Component: Cajal body; centrosome; chromosome, telomeric region; X chromosome; condensed chromosome; cytosol; Y chromosome; nucleoplasm; transcription factor complex; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus; endosome Molecular Function:cyclin binding; protein binding; cyclin-dependent protein kinase activity; metal ion binding; histone kinase activity; ATP binding Biological Process: G1 DNA damage checkpoint; meiosis; mitosis; positive regulation of transcription, DNA-dependent; histone phosphorylation; DNA repair; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; peptidyl-serine phosphorylation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; positive regulation of cell proliferation; Ras protein signal transduction; mitotic cell cycle; DNA replication; G2/M transition of mitotic cell cycle; blood coagulation; centrosome duplication; potassium ion transport; G1/S transition of mitotic cell cycle; positive regulation of DNA replication initiation
NCBI Summary:	This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
UniProt Code:	P24941
NCBI GenInfo Identifier:	116051
NCBI Gene ID:	1017
NCBI Accession:	P24941.2
UniProt Secondary Accession:	P24941,O75100, A8K7C6,
UniProt Related Accession:	P24941
Molecular Weight:	30,035 Da
NCBI Full Name:	Cyclin-dependent kinase 2
NCBI Synonym Full Names:	cyclin-dependent kinase 2
NCBI Official Symbol:	CDK2
NCBI Official Synonym Symbols:	CDKN2; p33(CDK2)
NCBI Protein Information:	cyclin-dependent kinase 2; p33 protein kinase; cdc2-related protein kinase; cell division protein kinase 2
UniProt Protein Name:	Cyclin-dependent kinase 2
UniProt Synonym Protein Names:	Cell division protein kinase 2; p33 protein kinase
Protein Family:	Cyclin-dependent kinase
UniProt Gene Name:	CDK2
UniProt Entry Name:	CDK2_HUMAN

Antibodies	ELISA Kits
Anti-CDK2 Antibody (CAB0294)	Human Cdk2 ELISA Kit
Anti-CDK2 Antibody (CAB16885)	Human CDK2 (Cyclin Dependent Kinase 2) CLIA Kit (HUES01200)
Anti-Phospho-CDK2-T160 Antibody (CABP0325)	Human Cyclin-dependent kinase 2 (CDK2) ELISA Kit
Anti-CDK2 Antibody (CAB0094)	CDK2 Colorimetric Cell-Based ELISA Kit
Anti-Phospho-CDK2-Y15 Antibody (CABP1005)	CDK2 (Phospho-Thr160) Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)