Anti-Acetyl-Histone H2A type 1-B/E (K9) Antibody (RACO0015)

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SKU:
RACO0015
Product type:
Recombinant Antibody
Reactivity:
Human
Host Species:
Human
Isotype:
IgG
Application:
IF
Application:
ELISA
Application:
ICC
Conjugation:
Unconjugated
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Description

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商品名:Acetyl-Histone H2A type 1-B/E (K9) Antibody
Product SKU:RACO0015
サイズ:50ul
宿主種:Human
申し込み:ELISA, ICC, IF
推奨される希釈:ICC:1:50-1:500, IF:1:30-1:200
反応性:Human
免疫原:A synthesized peptide
憲法:Liquid
ストレージバッファ:Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
精製方法:Affinity-chromatography
抗体のクローン性:Monoclonal
アイソタイプ:Rabbit IgG
Conjugate:Non-conjugated
バックグラウンド:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
シノニム:Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, HIST1H2AB, H2AFM, AND, HIST1H2AE, H2AFA
Product ImageImmunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) with RACO0015 at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Product ImageImmunocytochemistry analysis of RACO0015 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
UniProt Protein Function:HIST1H2AE: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H2A family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: nuclear chromatin; nucleus

Molecular Function:DNA binding

Biological Process: chromatin silencing; negative regulation of cell proliferation

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:P04908
NCBI GenInfo Identifier:124028530
NCBI Gene ID:3012
NCBI Accession:P04908.2
UniProt Secondary Accession:P04908,P28001, Q76P63,
UniProt Related Accession:P04908
Molecular Weight:14kDa
NCBI Full Name:Histone H2A type 1-B/E
NCBI Synonym Full Names:histone cluster 1 H2A family member e
NCBI Official Symbol:HIST1H2AE  
NCBI Official Synonym Symbols:H2A.1; H2A.2; H2A/a; H2AFA  
NCBI Protein Information:histone H2A type 1-B/E
UniProt Protein Name:Histone H2A type 1-B/E
UniProt Synonym Protein Names:Histone H2A.2; Histone H2A/a; Histone H2A/m
UniProt Gene Name:HIST1H2AB  
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